We report the development of a platform of dual targeting Fab (DutaFab) molecules, which comprise two spatially separated and independent binding sites within the human antibody CDR loops: the so-called H-side paratope encompassing HCDR1, HCDR3 and LCDR2, and the L-side paratope encompassing LCDR1, LCDR3 and HCDR2. Both paratopes can be independently selected and combined into the desired bispecifWe report the development of a platform of dual targeting Fab (DutaFab) molecules, which comprise two spatially separated and independent binding sites within the human antibody CDR loops: the so-called H-side paratope encompassing HCDR1, HCDR3 and LCDR2, and the L-side paratope encompassing LCDR1, LCDR3 and HCDR2. Both paratopes can be independently selected and combined into the desired bispecif

När skottskogsbruket/stubbskottsängsbruket upphörde i början av 1800-talet försvann även landskapet som humlesugan, Betonica officinalis L., var anpassad till. Från att ha varit allmän på sydvästra Lolland och i västra Mellanskåne blev den mycket sällsynt inom dessa kärnområden i humlesugans tidigare utbred- ning i Skandinavien. Ändå lyckades den överleva på sju lokaler. För att bevara dessa krävsWhen coppice forestry and the use of wooded meadows ceased in the early 19th century, the landscape to which Betony, Betonica officinalis L., was adapted also disappeared. From being common in southwestern Lolland and in western Mid­Skåne, it became very rare in these core areas of Betony’s earlier distribution in Scandi­ navia. However, the species has managed to survive at seven localities and t

OBJECTIVE: FLASH irradiation reportedly produces less normal tissue toxicity, while maintaining tumour response. To investigate oxygen's role in the 'FLASH effect', we assessed DNA damage levels following irradiation at different oxygen tensions, doses and dose rates.METHODS: Samples of whole blood were irradiated (20 Gy) at various oxygen tensions (0.25-21%) with 6 MeV electrons at dose rates of

Introduction. According to the 2021 ERC/ESICM guideline recommen-dations, elevated neuron-specific enolase [NSE] levels as well as diffuseand extensive anoxic damage on neuroimaging are predictors of poorneurological outcome after cardiac arrest.(1) We previously describedthat NSE is elevated in patients with generalised oedema on com-puted tomography [CT]. (2).Objectives. In this study, we aim to

The Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) is a dual-hemisphere, near-infrared (NIR), spectroscopic survey with the goal of producing a chemodynamical mapping of the Milky Way. The targeting for APOGEE-2 is complex and has evolved with time. In this paper, we present the updates and additions to the initial targeting strategy for APOGEE-2N presented in Zasowski et al.

While wealth-holding patterns in rural areas have been well studied, the link between initial conditions, prospects for wealth accumulation, and the persistence of inequality at an agricultural frontier is less clear. On the one hand, the frontier is thought to have had a levelling effect, with the availability of cheap land acting as an equalizer. On the other hand, land rents, accumulated during

Background: Essential proteins play an important role in the process of life, which can be identified by experimental methods and computational approaches. Experimental approaches to identify essential proteins are of high accuracy but with the limitation of time and resource-consuming. Objective: Herein, we present a computational model (PEPRF) to identify essential proteins based on machine lear

The post-Soviet Ukrainian polity has invested considerableresources into forming and managing what it refers to as “nationalmemory” (natsional’na pam’iat’). The Soviet forms and roots of thismemory production are easily discernible, though the ideologicalcontent has changed. Two presidents stood out as particularly activein the field of memory management: Viktor Yushchenko (2005–2010)and Petro Por

ocusing particularly on Poland and Ukraine, with less detailed considerations of other parts of eastern Europe, this chapter examines the politicization of Holocaust memory in the post-Cold War period. An attempt to forge a new, postcommunist identity in eastern Europe also entailed an evasion of wartime reality. The all too real suffering of Poles or Ukrainians during the Second World War was con

Heatwaves exert disproportionately strong and sometimes irreversible impacts on forest ecosystems. These impacts remain poorly understood at the tree and species level and across large spatial scales. Here, we investigate the effects of the record-breaking 2018 European heatwave on tree growth and tree water status using a collection of high-temporal resolution dendrometer data from 21 species acr

Carbon pricing has now been implemented as a key policy instrument for climate change mitigation in many countries globally. However, existing systems differ significantly in design with regard to stringency, coverage and the use of the revenues collected through the system. In this literature study, we synthesise existing knowledge of how key factors affect the adoption, implementation, and desig

The purpose of this article is to contribute to a refined perspective on how the practices of everyday life can challenge existing spatial scale relations, as well as produce new ones, and how this in turn can be addressed by planning. The investigation is based on a discussion of empirical studies dealing with the role of migrants in processes of place-making and urban transformation. In the arti

Background: Ambient particulate matter is a leading risk factor for disease globally. Particulate matter 10 (PM10) and particulate matter 2.5 (PM2.5) are derived from different sources, including operating motor vehicles as well as from industrial activities. In this study we investigate the association between increased concentrations of PM and total daily visits to the psychiatric emergency unit

Time-lapse resistivity surveys are commonly used to monitor temporal changes in the subsurface. In certain cases, it is known from other information that the resistivity will only decrease or increase with time. The 4-D resistivity smoothness-constrained inversion method reduces artifacts due to noise by including a temporal roughness filter constraint that ensures the temporal changes vary in a s

This paper studies the underlying mechanisms to binder phase lamella formation (in WC/WC grain boundaries) in hardmetal inserts used in face turning. A distribution of lamellae orientations has been quantified for used turning inserts as well as pristine inserts and coupled to the direction of the applied stress, calculated by FEM. Furthermore, more detailed FEM calculations on the micro scale hav

Transport energy conservation research in urban transport systems dates back principally to the Organization of the Petroleum Exporting Countries’ (OPEC) “Arab Oil Embargo” (1973–1974) and the Iranian revolution (1979), when global oil supplies became threatened and costs rose steeply. Two subsequent Gulf Wars (1991 and 2003) highlighted the dangerous geo-political dimensions of Middle-Eastern oil

Many democracies find it difficult to act swiftly on problems such as climate change and biodiversity loss. This is reflected in long-standing debates in research and policy about whether democratic practices are capable of fostering timely, large-scale transformations towards sustainability. Drawing on an integrative review of scholarly literature from 2011 to early 2021 on sustainability transfo

The connection between branch and trunk in a tree must be strong enough to transfer all loads acting on the branch, and it is well known that such branch-stem connections are indeed very strong. In this paper, X-ray computer tomography is employed to investigate the local fibre orientation in the close surrounding of a knot in a Norway spruce specimen to better understand the origins of the mechan

Background: Topical probiotics have been suggested as a treatment option for allergic rhinitis, as they may skew the immune response towards a beneficial type-1 non-allergic profile. So far observations in man have exclusively involved oral intake. The aim of this study was to examine whether a topical/nasal administration of a probiotic assemblage (PA) affects quality of life, symptoms and signs