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Manual cell counting is time consuming and requires a high degree of skill on behalf of the person performing the count. Here we use a technique that utilizes digital holography, allowing label-free and completely non-invasive cell counting directly in cell culture vessels with adherent viable cells. The images produced can provide both quantitative and qualitative phase information from a single

The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growi

Smoking is the main risk factor for COPD (chronic obstructive pulmonary disease) but genetic factors are of importance, since only a subset of smokers develops the disease. Sex differences have been suggested both in disease prevalence and response to environmental exposures. Furthermore, it has been shown that acquisition of 'addiction' to smoking is partly genetically mediated. Disease cases and

Nursing homes are a setting in which death and dying is common. How death and dying is articulated and the actions that take place in a nursing home constitute a discourse that guides the staff in their work. The aim of this study was to explore the discourse of death and dying in nursing homes from the perspective and understanding of the staff. The study draws on Foucault’s discourse analysis. D

Lack of recovery from everyday strains and demands is connected to increased levels of stress-related problems and development of ill-health. There is much research on how to handle and prevent fatigue and stress in leisure time, but research on working time is more scarce. A recent study found that employees' experience of recovery during working hours was related to high self-rated health. OBJEC

Walking as a means of transportation is a key feature in sustainable urban design, but few studies have examined the influence of micro-level environmental features, such as vegetation and street lighting, on perceived safety and people’s choice to walk. This study applied a non-explicit approach to examine the relationship between greenery, street lighting, perceived safety, and walking in an urb

Inherited mutations in the tumor suppressor genes BRCA1 and BRCA2 predispose carriers to breast and ovarian cancer. The authors have identified a mutation in BRCA2, 7845+1G > A (c.7617+1G > A), not previously regarded as deleterious because of incorrect mapping of the splice junction in the originally published genomic reference sequence. This reference sequence is generally used in many laborator

Type 1 diabetes (T1D) and other autoimmune diseases (AIDs) often co-occur in families. Leveraging data from 58,284 family trios in Finnish nationwide registers (FinRegistry), we identified that, of 50 parental AIDs examined, 15 were associated with an increased T1D risk in offspring. These identified epidemiological associations were further assessed in 470,000 genotyped Finns from the FinnGen stu

In this study of human carotid endarterectomy plaques (using 1128 regions of interest in a total of 10 plaques), we found that photon counting computed tomography (PCCT) can differentiate between attenuation and the spectral patterns of calcium, intra-plaque haemorrhage, thrombus, fibrosis, necrosis and lipid core. This is the first study to show the potential of PCCT in living patients using clin

This article seeks to contribute to understanding the practical “how” of a just climate transition as a systemic change with a focus on the local level. With the backdrop of the central role of municipalities in terms of governance in achieving climate transition while also fulfilling societal functions, it explores cases of eco-social innovations (ESI) of circular practices, with a specific inter