NMR STUDIES ON STRUCTURE AND DYNAMICS OF TWO-DOMAIN PROTEINS
Popular Abstract in Swedish Jag har under min forskarutbildning vid Lunds tekniska högskola studerat proteiner med hjälp av en metod som kallas kärnmagnetisk resonans, NMR. I levande organismer regleras allt liv av tre typer av molekyler: DNA, RNA och proteiner. Den centrala dogmen i biokemi "DNA-RNA-Protein" innebär att den genetiska kod som ärvs i form av DNA och som finns i varje cell i kroppeThe general objective of the present thesis work was to examine 3-dimensional structure as well as the motional properties of two different types of two-domain proteins by solution-state NMR. One of the major tasks of this thesis work was to investigate the domain orientation of these molecules by employing residual dipolar couplings (RDC) which can be used as orientational restraints for increasi