Formation of amyloid-like fibrils upon limited proteolysis of bovine alpha-lactalbumin
Bovine alpha-lactalbumin (alpha-LA) (10 g L-1) was incubated with a protease from Bacillus licheniformis at pH 7.5 and 50 degreesC. The reaction was biphasic consisting of an initial hydrolysis of intact alpha-LA and formation of dimers from large hydrolysis products within 60 min followed by aggregation of dimers into aggregates of 500 kDa. The aggregates consisted primarily of fibrillar strands
