A label-free high-throughput protein solubility assay and its application to Aβ40
A major hallmark of Alzheimer's disease is the accumulation of aggregated amyloid peptide (Aβ) in the brain. Here we develop a solubility assay for proteins and measure the solubility of Aβ40. In brief, the method utilizes 96-well filter plates to separate monomeric Aβ from aggregated Aβ, and the small species are quantified with the amine reactive dye o-phthalaldehyde (OPA). This procedure ensure