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Molekylär medicin: Molekylärgenetisk metodik avslöjar mekanismerna bakom tumörutveckling

The rapid advance in our understanding of cancer biology during the past decade, as exemplified by the discovery of oncogenes and tumour suppressor genes and their interactions in tumourigenesis, has revolutionized cancer research. This rapid progress has largely been due to the use of molecular genetics techniques. However, despite the wealth of available information as to the genetic basis of ca

Bioelectrocatalysis of plant peroxidases immobilized on graphite in aqueous and mixed solvent media

The bioelectrocatalytic function of two plant peroxidases, horseradish peroxidase (HRP) and a newly purified royal palm tree-leaf peroxidase (RPTP), was studied on graphite electrodes in aqueous buffer solutions, over the pH range 7.0 - 3.0, and in aqueous buffer solutions, at pH 6.0, containing different amounts of a polar organic cosolvent, i.e., ethanol and acetonitrile. The kinetics of direct

Performance evaluation of list sequence MAP decoding

List-sequence (LS) decoding has the potential to yield significant coding gain additional to that of conventional single-sequence decoding, and it can be implemented with full backward compatibility in systems where an error-detecting code is concatenated with an error-correcting code. LS maximum-likelihood (ML) decoding provides a list of estimated sequences in likelihood order. For convolutional

A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets.

Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation tec

Evasive response to ultrasound by the crepuscular butterfly Manataria maculata

The crepuscular nymphalid butterfly Manataria maculata was studied in Monteverde cloud forest, Costa Rica, during the dry season reproductive diapause. M. maculata has ears in the form of Vogel's organs located near the base of the forewings. Its behaviour in response to bursts of ultrasonic pulses (26 kHz, 110 dB SPL at 1 m) was condition-dependent. At dusk and dawn the sound consistently elicite

Shot noise in tunneling through a single quantum dot

We investigate the noise properties of a zero-dimensional InAs quantum dot (QD) embedded in a GaAs-AlAs-GaAs tunneling structure. We observe an approximately linear dependence of the Fano factor and the current as a function of bias voltage. Both effects can be linked to the scanning of the three-dimensional emitter density of states by the QD. At the current step the shape of the Fano factor is m

Cretaceous palynofloras from southern Scandinavia

Popular Abstract in Swedish Avhandlingen omfattar studiet av fossila pollen och sporer (palynologi) från skånes krita (90-140 miljoner år gamla sediment). För ändamålet har tre borrkärnor provtagits; Höllviken 2, Norrevång 1 och Svedala 1, samt ytprover från överkritan på Bornholm. RESULTAT * Taxonomiska studier baserade på ca 100 arter (ca. 15 000 exemplar), har möjliggjort en stratigrafisk indeA palynological investigation of Cretaceous sequences from Höllviken 2, Svedala 1 and Norrevång 1 wells of Scania, Southern Sweden, and from the Arnager Greensand-Arnager Limestone transition on the island of Bornholm, Denmark, was conducted. Well preserved palynofloras were found in strata consisting of shaly mudstones, glauconitic sandstones (greensand) and limestone. A total of 98 palynomorph t

Jämviktsprovtagning

Jämviktsprovtagning genom membran är ett nytt koncept där porösa hålfibrer används för upprening, anrikning och mätning av polära organiska föreningar och metaller. Metoden kan användas såväl i laboratoriet som till fältprovtagning

Differential impairment of glucagon responses to hypoglycemia, neuroglycopenia, arginine, and carbachol in alloxan-diabetic mice.

To gain insight into the mechanisms responsible for the loss of the glucagon response to insulin-induced hypoglycemia in type 1 diabetes, glucagon responses to 4 different stimuli were examined over 3 months of diabetes in alloxan-treated mice. At 1, 6, and 12 weeks after alloxan (60 mg/kg), phloridzin (0.1 g/kg) was administered to overnight fasted diabetic mice to match the glucose levels of tho

Comparative study of methyl-CpG-binding domain proteins

BACKGROUND: Methylation at CpG dinucleotides in genomic DNA is a fundamental epigenetic mechanism of gene expression control in vertebrates. Proteins with a methyl-CpG-binding domain (MBD) can bind to single methylated CpGs and most of them are involved in transcription control. So far, five vertebrate MBD proteins have been described as MBD family members: MBD1, MBD2, MBD3, MBD4 and MECP2. RESULT

Accurate time-resolved laser spectroscopy on lithium atoms

The lifetime of the lithium 2p2P states has been measured with high accuracy using the delayed coincidence technique with a continuous mode-locked dye laser as the source of the excitation light. The value 27.22 (0.20) ns was obtained. In addition, the hyperfine structure of the7Li 2p2P3/2 state, which can normally scarcely be resolved, has been studied by recording the slow quantum beats at zero

Comparison between tower and aircraft-based eddy covariance fluxes in five European regions

Airborne eddy covariance measurements provide a unique opportunity to directly measure surface energy, mass and momentum fluxes at the regional scale. This offers the possibility to complement the data that are obtained by the ground-based eddy covariance networks and to validate estimates of the surface fluxes that can be obtained by means of satellite products and models. The overall accuracy an