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Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip.
Due to the extensive use of nucleic acid and protein analysis of bacterial samples, there is a need for simple and rapid extraction protocols for both plasmid DNA and RNA molecules as well as reporter proteins like the green fluorescent protein (GFP). In this report, an electropermeability technique has been developed which is based on exposing E. coli cells to low voltages to allow extraction of
Attosecond Control in Photoionization of Hydrogen Molecules
We report experiments where hydrogen molecules were dissociatively ionized by an attosecond pulse train in the presence of a near-infrared field. Fragment ion yields from distinguishable ionization channels oscillate with a period that is half the optical cycle of the IR field. For molecules aligned parallel to the laser polarization axis, the oscillations are reproduced in two-electron quantum si
Effects of color reconnection on t(t)over-bar final states at the LHC
The modeling of color reconnection has become one of the dominant sources of systematic uncertainty in the top mass determination at hadron colliders. The uncertainty on the top mass due to color reconnection is conventionally estimated by taking the difference in the predictions of a model with and a model without color reconnection. We show that this procedure underestimates the uncertainty when
Improvements to an Empirical Parameterization of Heterogeneous Ice Nucleation and Its Comparison with Observations
A framework for an empirical parameterization (EP) of heterogeneous nucleation of ice crystals by multiple species of aerosol material in clouds was proposed in a 2008 paper by the authors. The present paper reports improvements to specification of a few of its empirical parameters. These include temperatures for onset of freezing, baseline surface areas of aerosol observed in field campaigns over
Discovery of biomarkers for glycaemic deterioration before and after the onset of type 2 diabetes: rationale and design of the epidemiological studies within the IMI DIRECT Consortium.
The DIRECT (Diabetes Research on Patient Stratification) Study is part of a European Union Framework 7 Innovative Medicines Initiative project, a joint undertaking between four industry and 21 academic partners throughout Europe. The Consortium aims to discover and validate biomarkers that: (1) predict the rate of glycaemic deterioration before and after type 2 diabetes onset; (2) predict the resp
Difficulties and pitfalls in the laboratory diagnosis of bleeding disorders
. von Willebrand disease (VWD) is the most common inherited bleeding disorder, but variable severity and several classification types mean that diagnosis is often not straightforward. In many countries, the assays are not readily available and/or are not well standardized. The latest methods and the basis of VWD are discussed here, together with information from the international quality assessmen
Contact allergies to potential allergens in patients with oral lichen lesions.
OBJECTIVE: The aim of the present controlled study was to investigate a possible relationship between contact allergies to potential allergens and oral lichen lesions. METHODS: Eighty-three patients with oral lichen lesions (OLL) and control groups of age- and gender-matched dermatitis patients (DP, n = 83) and patch-tested dermatitis patients randomly selected from files (PSFF, n = 319) were incl
Surface Chemistry of Alanine on Ni{111}
The adsorption of l-alanine on Ni{111} has been studied as a model of enantioselective heterogeneous catalysts. Synchrotron-based X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure (NEXAFS) spectroscopy were used to determine the chemical state, bond coordination, and out-of-plane orientation of the molecule on the surface. Alanine adsorbs in anionic and zwitterionic fo
No specific effect of whole-body vibration training in chronic stroke: a double-blind randomized controlled study.
OBJECTIVE: To evaluate the effects of whole-body vibration (WBV) training in individuals after stroke. DESIGN: A double-blind randomized controlled study with assessments pre- and posttraining. SETTING: A university hospital rehabilitation department. PARTICIPANTS: Participants (N=31; mean age ± SD, 62±7y; 6-101mo poststroke) were randomized to an intervention group or a control group. INTERVE
Thrombin generation in abdominal sepsis is Rho-kinase-dependent.
Sepsis causes severe derangements of the coagulation system. However, the signaling mechanisms regulating sepsis-induced thrombin generation remain elusive. Herein, we hypothesized that Rho-kinase might be an important regulator of thrombin generation in abdominal sepsis. Abdominal sepsis was induced by cecal ligation and puncture (CLP) in C57Bl/6 mice. Thrombin generation, coagulation factors, lu
Accuracy of a Panel of 5 Cerebrospinal Fluid Biomarkers in the Differential Diagnosis of Patients With Dementia and/or Parkinsonian Disorders
Objective: To assess the ability of 5 cerebrospinal fluid (CSF) biomarkers to differentiate between common dementia and parkinsonian disorders. Design: A cross-sectional, clinic-based study. Participants: Cerebrospinal fluid samples (N=453) were obtained from healthy individuals serving as controls and from patients with Parkinson disease (PD), PD with dementia (PDD), dementia with Lewy bodies (DL
Polymer Adsorption from Bulk Solution onto Planar Surfaces: Effect of Polymer Flexibility and Surface Attraction in Good Solvent
Adsorption of uncharged homopolymers of various flexibilities in good solvent onto planar surfaces Lit various polymer-surface interaction strengths have been investigated by employing a coarse-grained bead-spring polymer model using simulation techniques. The polymer flexibility ranged from fully flexible to rod-like polymers, and the adsorption strength varied from weak to strong adsorption. Equ
Long-term Results of Cardiac Resynchronization Therapy; A Comparison Between CRT-Pacemakers Versus Primary Prophylactic CRT-Defibrillators.
Cardiac resynchronization Therapy (CRT) with or without a defibrillator has a positive effect on mortality and morbidity for patients with heart failure. However, comparisons between CRT-Defibrillators (CRT-D) and CRT-Pacemakers (CRT-P) are relatively scarce outside the clinical trial setting. This study aimed to assess baseline characteristics in relation to long-term prognosis in patients treate
Having to focus on doing rather than being-Nurse assistants' experience of palliative care in municipal residential care settings.
BACKGROUND: Palliative care should be provided, irrespective of setting to all patients facing a life-threatening illness and to their families. The situation and needs of older people differ from those of younger people since they often have several co-existing diseases and health complaints. This implies an extensive need for care and for longer periods of palliative care. The main providers of
Soot reference materials for instrument calibration and intercomparisons: a workshop summary with recommendations
Soot, which is produced from biomass burning and the incomplete combustion of fossil and biomass fuels, has been linked to regional and global climate change and to negative health problems. Scientists measure the properties of soot using a variety of methods in order to quantify source emissions and understand its atmospheric chemistry, reactivity under emission conditions, interaction with solar
Characterizing the Composition of Bone Formed During Fracture Healing Using Scanning Electron Microscopy Techniques.
About 5-10 % of all bone fractures suffer from delayed healing, which may lead to non-union. Bone morphogenetic proteins (BMPs) can be used to induce differentiation of osteoblasts and enhance the formation of the bony callus, and bisphosphonates help to retain the newly formed callus. The aim of this study was to investigate if scanning electron microscopy (SEM) and energy-dispersive X-ray spectr
Evaluation of metal artifacts in clinical MR images of patients with total hip arthroplasty using different metal artifact-reducing sequences.
To evaluate the distortion and artifact area of metal in MR images and to compare artifact reduction using different metal artifact-reducing sequences in patients with metal-on-metal (MoM) and non-MoM total hip prostheses.
Improved one-step steam pretreatment of SO2-impregnated softwood with time-dependent temperature profile for ethanol production.
In the production of ethanol from lignocellulosic material, pretreatment of the raw material before enzymatic hydrolysis and fermentation is essential to obtain high overall yields of sugar and ethanol. Two-step steam pretreatment results in higher ethanol yields from softwood than the standard one-step pretreatment process. However, the difficulty with separation and washing of the material at hi
Genetic variation in alcohol dehydrogenase (ADH1A, ADH1B, ADH1C, ADH7) and aldehyde dehydrogenase (ALDH2), alcohol consumption and gastric cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort
Studies that have examined the association between alcohol consumption and gastric cancer (GC) risk have been inconsistent. We conducted an investigation of 29 genetic variants in alcohol metabolism loci (alcohol dehydrogenase, ADH1 gene cluster: ADH1A, ADH1B and ADH1C; ADH7 and aldehyde dehydrogenase, ALDH2), alcohol intake and GC risk. We analyzed data from a nested case-control study (364 cases