Application of molecular barcoding and engineered viral vectors for the determination of causative transcriptomic changes in Parkinson’s disease and generation of a fluorescent reporter model for studying cell-cell communication in the brain
Parkinson’s disease (PD) is a neurodegenerative disorder with toxic forms of alpha synuclein (α-syn) accumulating in the midbrain dopaminergic (DA) neurons, leading to cell death. In the first part of the study we wanted to investigate the midbrain DA population and their gene expression relative to the intracellular α-syn load. To this end, human wildtype α-syn was packaged inside custom, uniquel