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We report on the use of aqueous two-phase systems for the side-chain degradation of cholesterol into androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD) by Mycobacterium sp. A problem encountered at the start of this investigation, extensive aggregation of the cells, was solved by careful choice of the polymer and detergent composition of the phase system. The systems showing the
We describe a new method for quantitatively measuring substances of clinical interest by high-performance liquid affinity chromatography (HPLAC). As a model system we selected analysis for transferrin in human serum with immobilized antibodies in a high-performance liquid chromatographic system. SelectiSpher-10 Activated Tresyl columns (5 or 10 x 0.5 cm) were used for in situ coupling of poly
Due to their conceptual appeal and computational convenience, two-level systems (TLSs) and generalizations are used to investigate nonlinear behavior in quantum optics and assess the applicability of theoretical methods. Herein, the focus is on second-harmonic generation (SHG) and, as system of interest, on the Dicke model, which consists of several TLSs inside an optical cavity. The main aspect a
Background: Remaining pain and functional limitations may cause dissatisfaction in patients after total knee arthroplasty (TKA). Furthermore, anxiety and depression are associated with remaining postoperative symptoms and dissatisfaction. We investigated if patient-reported anxiety or depression increased the risk of dissatisfaction 1 year after TKA in patients who improved in pain or function. Me
A new method for preparing chromatographic supports is described. Porous silica was treated with gaseous silanes and gaseous triethylamine at high temperature and reduced pressure. The silylation procedure was rapid and gave supports with a covalently bound monolayer of aminopropyl-or epoxysilane. Diol silica prepared by gas phase silylation was compared with diol silica prepared in the aqueous or
Syftet med den här licentiatuppsatsen är att ur ett universell utformningsperspektiv undersöka vad som kännetecknar dagens återvinningsmiljöer i kommunala bostadsområden och vilka värden som finns kopplade till dessa. De två frågeställningarna är: Vilka normer är framträdande i urvalet återvinningsmiljöer och hur bidrar utformningen till upplevelsen av inkluderande återvinningsmiljöer? Den första The aim of this licentiate thesis is to examine from a universal design perspective whatcharacterizes today's recycling environments in residential areas and what values are linked to them.The first study was carried out at a local level in a recycling environment in a municipality in Norrland.The method used on site was go along-studies and this involved professionals from the municipalrecycling
Severe chronic rhinosinusitis with nasal polyposis (CRSwNP) is a disabling airway disease that significantly impacts patients’ lives through the severity of symptoms, the need for long-term medical treatment and the high risk of recurrence post-surgery. Biological agents targeting type 2 immune responses underlying the pathogenesis of CRSwNP have shown effectiveness in reducing polyp size and eosi
Mycobacterium sp. (NRRL-B 3683) has been immobilized by adhesion of magnetic materials of submicron size to the bacterial surface. Preparations based on laboratory-prepared magnetic oxide that had been derivatized with hydrophobic octyltrichlorosilane showed the best properties. The magnetically immobilized bacteria were used for side-chain degradation of cholesterol into androsta-1,4-diene-3,17-d
The autonomy created for Åland in 1920 along with the linguistic and cultural guarantees stipulated in the 1921 Åland Agreement established Åland as a monolingual Swedish-speaking province within otherwise bilingual Finland. In particular, the Ålandic municipalities could themselves decide whether Finnish was to be taught in their primary schools. One hundred years later, Åland is still monolingua
Objective: The association between work-related moderate-to-vigorous physical activity (MVPA) and higher levels of stress response is recognized, but whether this association is moderated by regular exercise remains unclear. This cross-sectional study investigated whether exercise-based physical activity (PA) associates with lower levels of stress responses moderated by work-related MVPA. Methods:
Introduction: The CVA24 antigenic variant is the causal agent of epidemics of acute hemorrhagic conjunctivitis that has prevailed to date. To study the transmission dynamics and evolution of this variant, we molecularly characterized Cuban isolates obtained in 5 epidemic periods between 1986 and 2009. Methods: Using the BEAST v1.8.4 program, it was performed the phylogenetic and phylodynamic analy
A screening of immobilized α-chymotrypsin preparations suitable for the synthesis of N-acetyl-L-tyrosine ethyl ester from N-acetyl-L-tyrosine and up to 99% ethanol was carried out. α-Chymotrypsin adsorbed to Sepharose LH-20 or covalently bound to Sepharose 4B (tresyl chloride activation) was found to be an efficient catalyst. A column packed with immobilized enzyme retained 60% of its initial acti
Diabetes remains one of the four major causes of morbidity and mortality globally among non-communicable diseases (NCDs. It is predicted to increase in sub–Saharan Africa by over 50% by 2045. The aim of this study is to identify, map and estimate the burden of diabetes in Ghana, which is essential for optimising NCD country policy and understanding existing knowledge gaps to guide future research
Objectives Critical care is essential in saving lives of critically ill patients, however, provision of critical care across lower resource settings can be costly, fragmented and heterogenous. Despite the urgent need to scale up the provision of critical care, little is known about its availability and cost. Here, we aim to systematically review and identify reported resource use, availability and
Introduction Health technology assessment (HTA) is an area that remains less implemented in low- and lower middle-income countries. The aim of the study is to understand the perceptions of stakeholders in Uganda toward HTA and its role in decision making, in order to inform its potential implementation in the country. Methods The study takes a cross-sectional mixed methods approach, utilizing an a
A new type of support for chromatographic use has been developed. Non-porous quartz fibre, with a mean diameter of 0.5 μm, was silylated with mercaptopropyltrimethoxysilane. Tresyl chloride-activated dextran was covalently coupled to the SH groups on the fibre. Remaining active tresyl groups on the dextran were then coupled with an NAD derivative.The affinity fibre contained 0.3 μmol NAD derivativ
