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Diagnosis of tuberculous meningitis: a comparative analysis of 3 immunoassays, an immune complex assay and the polymerase chain reaction

OBJECTIVE: To compare 3 immunoassays, an immune complex assay, and an application of the polymerase chain reaction (PCR) for the diagnosis of tuberculous meningitis (TBM). MATERIAL: Cerebrospinal fluid (CSF) from 33 patients with TBM and from 34 control patients with infectious and non-infectious CNS diseases was analysed. RESULTS: The antibody immunoassays were either nonspecific or insensitive.

Forking and Unusual Decay Out of Superdeformed Bands in 83Zr

Two superdeformed (SD) bands extending over eight to eleven transitions have been identified in Zr-83, The quadrupole moment of the more intense band was determined by the Residual Doppler Shift Method and is consistent with a quadrupole deformation of beta(2) approximate to 0.5. The large quadrupole moment and population intensity of the yrast SD band (approximate to 5%) in Zr isotopes relative t

Insulin-induced translocation of protein kinase B to the plasma membrane in rat adipocytes

Protein kinase B (PKB) has previously been shown to be activated in response to insulin and growth factor stimulation. The activation mechanism has been suggested to involve translocation of PKB to membranes, where it is phosphorylated and activated. Insulin-induced translocation of PKB has not been demonstrated in a physiological target cell. Therefore we have used the primary rat adipocyte to in

The early Nordic software effort

Early Nordic cooperation comprised ideas, programs, even computer blueprints and resulted in rapid progress. Regnecentralen contributed importantly in developing Algol 60. Algol for Facit EDB was used at many sites. Further advances were Datasaab's Algol Genius and the Norwegian Simula project. Regrettably, failure of our compiler companies led to software setbacks. We revisit the Algol debates co

Total column densities of tropospheric and stratospheric trace gases in the undisturbed Arctic summer atmosphere

Ground-based FTIR measurements have been performed in the Arctic summer in July 1993 and June 1994 at 79 degrees N to study the zenith column densities of several trace gases in the undisturbed Arctic summer atmosphere. Zenith column densities of H2O, N2O, HNO3, NO2, NO, ClONO2, ClO, HCl, HF, COF2, OCS, SF6, HCN, CH4, C2H6, C2H2, CO, O-3, CFC-12, CFC-22, and CO2 were retrieved by line-by-line calc

Hepatocellular carcinoma in Sweden: its association with viral hepatitis, especially with hepatitis C viral genotypes

Viral markers of chronic hepatitis were tested for in 95 frozen serum samples from 299 patients from Malmo, Sweden, with hepatocellular carcinoma (HCC), diagnosed between 1977 and 1994. Hepatitis B analysis included anti-HBc, HBsAg and, if anti-HBc positive, HBV DNA. Hepatitis C infection analysis included anti-HCV screening, RIBA, HCV RNA and HCV genotyping. HCV genotyping was also carried out in

Investigation of a two-equation turbulent heat transfer model applied to ducts

This investigation concerns numerical calculation of fully developed turbulent forced convective heat transfer and fluid flow in ducts over a wide range of Reynolds numbers. The low Reynolds number version of a non-linear eddy viscosity model is combined with a two-equation heat flux model with the eddy diffusivity concept. The model can theoretically be used for a range of Prandtl numbers or a ra

Plasmid purification using non-porous anion-exchange silica fibres

A new type of fibre-based anion-exchange material for plasmid purification was developed. The basic material consisted of non-porous silica fibres with a mean diameter of 1.5 mu m and a surface area of 2.4 m(2) g(-1). The fibre surface was provided with several types of ligands, either by adsorption of polymers (chitosan or poly(ethyleneinune)) or by polymerization of amine-containing acrylic mono

The influence of dietary nucleotides on erythrocyte membrane fatty acids and plasma lipids in preterm infants

OBJECTIVE: The objective of this study was to evaluate whether a regular formula for premature infants supplemented with nucleotides has any influence on plasma lipids and erythrocyte membrane fatty acids. METHODS: Preterm infants fed either human milk supplemented with human milk protein (HM, n = 14), nucleotide-supplemented preterm formula (NF, n = 13), or a regular preterm formula (F, n = 13) w

The high-fat diet-fed mouse: a model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes.

This study characterizes the high-fat diet-fed mouse as a model for impaired glucose tolerance (IGT) and type 2 diabetes. Female C57BL/6J mice were fed a high-fat diet (58% energy by fat) or a normal diet (11% fat). Body weight was higher in mice fed the high-fat diet already after the first week, due to higher dietary intake in combination with lower metabolic efficiency. Circulating glucose incr

Bijective image registration using thin-plate splines

Image registration is the process of geometrically aligning two or more images. In this paper we describe a method for registering pairs of images based on thin-plate spline mappings. The proposed algorithm minimizes the difference in gray-level intensity over bijective deformations. By using quadratic sufficient constraints for bijectivity and a least squares formulation this optimization problem

Circulating blood diminishes cement penetration into cancellous bone. In vivo studies of 21 arthrotic femoral heads

We compared the penetration depth into cancellous bone when pressurizing cement at predetermined pressure levels, and at different times after cement mixing, in 21 arthrotic femoral heads during total hip replacement. To determine the influence of circulating blood on cement penetration, cement was injected into holes drilled into the femoral head before and after osteotomy of the femoral neck. Th

Heat Flow in Building Components, Experiment and Analysis

Three building components exposed to natural climate were studied: a dynamic insulation in the ceiling of a house, an outer wall and one window in the same wall. The term dynamic insulation implies that part of the inlet or exhaust air passes through the insulation of a house. A house with dynamic insulation was continuously measured for approximately a year and a half. The performance of the dyna

A mechanical stretch induces contractile activation in unstimulated developing rat skeletal muscle in vitro

The effects of a stretch-release cycle (25 of the resting muscle fibre length, Lo) on both tension and [Ca2+i in small, unstimulated, intact muscle fibre bundles isolated from adult and neonatal rats were investigated at 20 C. The results show that the effects of the length change depended on the age of the rats. Thus, the length change produced three effects in the neonatal rat muscle fibre bundl

Protein tyrosine phosphorylation in the transition to light state 2 of chloroplast thylakoids

Redox dependent protein phosphorylation in chloroplast thylakoids regulates distribution of excitation energy between the two photosystems of photosynthesis, PS I and PS II. Several thylakoid phosphoproteins are known to be phosphorylated on N-terminal threonine residues exposed to the chloroplast stroma. Phosphorylation of light harvesting complex II (LHC II) on Thr-6 is thought to account for re