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Three pre-PCR processing strategies for the detection and/or quantification of Salmonella in naturally contaminated soy bean meal were evaluated.
Methacrylic acid was produced in high yield by an integrated process involving bioconversion of 2-methyl-1,3-propanediol (2M1,3PD) to 3-hydroxy-2-methylpropionic acid (3H2MPA) via 3-hydroxy-2-methylpropanal (3H2MPAL), and catalytic dehydration of the resulting acid. Whole cells of Gluconobacter oxydans grown on glycerol-based culture medium were used as the catalyst for oxidative biotransformation
The elucidation of the molecular mechanisms by which soluble proteins convert into their amyloid forms is a fundamental prerequisite for understanding and controlling disorders that are linked to protein aggregation, such as Alzheimer's and Parkinson's diseases. However, because of the complexity associated with aggregation reaction networks, the analysis of kinetic data of protein aggregation to
Objectives: Short relative telomere length (RTL) is associated with vascular ageing, inflammation and cardiovascular risk factors. Previous studies have reported an association between abdominal aortic aneurysm and short RTL. The presence of atherosclerosis among patients with aneurysm disease may, however, be a confounder. The aim was to explore the associations between short RTL and aneurysm dis
Attention-deficit/hyperactivity disorder (ADHD) predisposes to drug abuse (DA) and twin studies suggest shared genetic effects. We here seek to determine, using adoption and adoption-like samples, the magnitude of the cross-generational transmission from DA in parents to ADHD in their children and clarify the degree to which this arises from genetic v. rearing effects.
Regional groupings of a majority, or all, of Earth's crustal blocks have occurred several times in Earth history, but only the most recent supercontinent Paleozoic Pangea/Gondwana, is well characterized. Prior Precambrian supercontinents are postulated: Rodinia (ca. 1 to 0.7 Ga), Nuna/Columbia (ca. 1.8 to 1.3 Ga) and Kenorland/supercratons (ca. >2.7 to 2.0 Ga), but the configuration of each is poo
Ancient DNA trapped in the matrices of ceramic transport jars from Mediterranean shipwrecks can reveal the goods traded in the earliest markets. Scholars generally assume that the amphora cargoes of 5th-3rd century B.C. Greek shipwrecks contained wine, or to a much lesser extent olive oil. Remnant DNA inside empty amphoras allows us to test that assumption. We show that short 100 nucleotides of an
Germany is generally regarded as a case of qualitative “change” in minimum income protection (MIP) schemes, while Sweden is perceived as one of institutional “inertia”.This paper seeks to qualify this view by embedding developments in MIP in wider policy and governance trends. Empirically, it is based on document analysis and qualitative expert interviews in the two countries. Theoretically, the p
Abstract in UndeterminedPiece rate and performance based wage systems are common in the construction industry. Construction workers are known to have an increased risk of pain and musculoskeletal disorders (MSD). In this cross-sectional questionnaire study, we examined the association between wage system and (1) physical exertion, (2) time pressure, (3) pain, and (4) fatigue. The participants comp
An association between preterm birth and an increased risk for childhood asthma has been demonstrated but the importance of intrauterine growth retardation on asthma risk is unclear.Using data from Swedish health registers, infant characteristics and childhood asthma were studied. Analyses were made using Mantel-Haenszel methodology with adjustment for year of birth, maternal age, parity, smoking
The Ncf1 gene, encoding the P47PHOX protein that regulates production of reactive oxygen species (ROS) by the phagocyte NADPH oxidase complex, is associated with autoimmunity and arthritis severity in rats. We have now identified that the single nucleotide polymorphism (SNP) resulting in an M153T amino acid substitution mediates arthritis resistance and thus explains the molecular polymorphism und
Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived fr
AIMS/HYPOTHESIS: Protein kinase C (PKC) regulates exocytosis in various secretory cells. Here we studied intracellular translocation of the PKC isoenzymes PKCalpha and PKCdelta, and investigated how activation of PKC influences glucagon secretion in mouse and human pancreatic alpha cells. METHODS: Glucagon release from intact islets was measured in static incubations, and the amounts released were
In everyday life, people use language to communicate evaluative messages about social categories. We examine a selection bias in language across two social dimensions not previously integrated; a self-inclusive/self-exclusive dimension and an individual/collective dimension. We used pronouns as markers for social categories (I, We, He/she and They), and developed a new measure, the Evaluative Sent
Diets high in protein have shown positive effects on short-term weight reduction and glycaemic control. However, the understanding of how dietary macronutrient composition relates to long-term risk of type 2 diabetes is limited. The aim of the present study was to examine intakes of macronutrients, fibre and protein sources in relation to incident type 2 diabetes. In total, 27 140 individuals, age
P>1. Female preferences for particular male ornaments may shift between populations as a consequence of ecological differences that change the reliability and detectability of the ornament, but few studies have examined how ornaments function in different populations. 2. We examined the signalling function of male plumage ornaments in a warbler, the common yellowthroat (Geothlypis trichas), breedi
The multiple energy states of water held by surfaces of a clay mineral can be effectively probed with time-of-flight and fixed elastic window neutron scattering. We used these techniques to quantitatively differentiate water types, including rotational and translational diffusions, in Ca- and Na-montmorillonite (SAz-1) and charge-reduced preparations equilibrated at RH = 33% and 55%, whose gravime
