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Electrospraying of colloidal nanoparticles for seeding of nanostructure growth

Nanometre-sized particles (1-100 nm) have unique properties receiving growing attention in wide areas of research. Here, a convenient method to deposit size-selected nanoparticles on surfaces by means of electrospraying colloidal suspensions in the aerosol phase is presented. We demonstrate the deposition of individual nanoparticles and the feasibility of this method in seeding gold particles for

Allozyme variation in Carex sect. Digitatae - Evidence of introgression, genetic distinctiveness and evolution of taxa

A subset of populations of the European taxa of Carex sect. Digitatae, including Carex digitata, C. pallens, C. ornithopoda, C. pediformis ssp. rhizodes and C. humilis have been investigated for allozyme variation. The (presumably) distantly related C. pendula was used a's a reference taxon. Carex digitata, C. pallens and C. ornithopoda on the one hand, and C. humilis and C. pediformis on the othe

Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.

To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between

Implantable drainage after major abdominal surgery in compromised patients

The risk of superinfection following routine abdominal drainage after major surgery is debated. Especially in patients with malignant diseases and a compromised host defense, this might be a factor increasing morbidity and mortality. During a 3-year period (1986-1988) 41 patients operated on for malignant abdominal conditions received a peritoneal catheter connected to a subcutaneous portal insert

Prediction of silent celiac disease at diagnosis of childhood type 1 diabetes by tissue transglutaminase autoantibodies and HLA

Aims: The aims were to estimate the diagnostic sensitivity and specificity of autoantibodies to tissue transglutaminase (IgA- and IgG-tTG), gliadin (AGA) and endomysium (EMA) in relation to human leukocyte antigen (HLA)-DQB1 alleles to identify silent celiac disease at diagnosis of type 1 diabetes. Methods: IgA- and IgG-tTG were measured in radioligand binding assays in 165 type 1 diabetic patien

Temporally fluctuating prey and interfering predators: a positive feedback

Prey densities often show fluctuating patterns over various timescales. Focusing on short-term, within-generation fluctuating patterns of local prey availability, we suggest that prey that show synchronized and high-amplitude fluctuations in availability experience decreased risks of predation, but also enhance the maintenance of predator interference hierarchies by affecting the relative foraging

Effect of Cold Starvation, Acid Stress, and Nutrients on Metabolic Activity of Helicobacter pylori.

Helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called U forms. The importance of nonculturable coccoid forms of H. pylori in disease transmission and antibiotic treatment failures is unclear. Metabolic activities of actively growing as well as nonculturable H. pylori were investigated by compari

Purification and characterization of human intestinal neutral ceramidase.

Sphingolipids are degraded by sphingomyelinase and ceramidase in the gut to ceramide and sphingosine, which may inhibit cell proliferation and induce apoptosis, and thus have anti-tumour effects in the gut. Although previous rodent studies including experiments on knockout mice indicate a role of neutral ceramidase in ceramide digestion, the human enzyme has never been purified and characterized i

Plasma markers of inflammation and incidence of hospitalisations for COPD: results from a population-based cohort study

Background: The relationship between plasma markers of inflammation and the incidence of chronic obstructive pulmonary disease (COPD) is still unclear. This population-based study explored whether raised levels of five inflammation-sensitive plasma proteins (ISPs) predicted hospital admissions for COPD during 25 years of follow-up. Methods: Spirometric tests and measurements of five ISPs (fibrinog

Ultrasensitive mass sensor fully integrated with complementary metal-oxide-semiconductor circuitry

Nanomechanical resonators have been monolithically integrated on preprocessed complementary metal-oxide-semiconductor (CMOS) chips. Fabricated resonator systems have been designed to have resonance frequencies up to 1.5 MHz. The systems have been characterized in ambient air and vacuum conditions and display ultrasensitive mass detection in air. A mass sensitivity of 4 ag/Hz has been determined in

Local maxima of Gaussian fields

The structure of a stationary Gaussian process near a local maximum with a prescribed heightu has been explored in several papers by the present author, see [5]–[7], which include results for moderateu as well as foru→±∞. In this paper we generalize these results to a homogeneous Gaussian field {ξ(t) t ∈ R n}, with mean zero and the covariance functionr(t). The local structure of a Gaussian field

Prevention of severe contractures might replace multilevel surgery in cerebral palsy: results of a population-based health care programme and new techniques to reduce spasticity

During the 1990s three new techniques to reduce spasticity and dystonia in children with cerebral palsy (CP) were introduced in southern Sweden: selective dorsal rhizotomy, continuous intrathecal baclofen infusion and botulinum toxin treatment. In 1994 a CID register and a health care programme, aimed to prevent hip dislocation and severe contractures, were initiated in the area. The total populat

BCG scar and positive tuberculin reaction associated with reduced child mortality in West Africa. A non-specific beneficial effect of BCG?

Previous studies have suggested that the bacille Calmette-Guerin (BCG) vaccine may have a non-specific beneficial effect on childhood survival in areas with high mortality. We examined whether BCG-vaccinated children with a BCG scar or a positive tuberculin reaction had better survival than children without such reactions. As part of an ongoing two-dose measles vaccine trial for which children wer

Studies on energy dissipation in phycobilisomes using the Kennard-Stepanov relation between absoption and fluorescence emission spectra

Absorption and fluorescence emission spectra were measured at room temperature (ca. 22.degree.C) for solutions of phycocyanin-1, phycocyanin-2, and allophycocyanin from Phormidium luridum, and also for phycobilisome preparations from various blue-green algae (Anabaena variabilis, Nostoc muscorum strain A, Nostoc sp. strain Mac. Phormidium luridum). Kennard-Stepanov (KS) temperatures (T) were compu

Relationship between the physical shape and the efficiency of oligomeric chitosan as a gene delivery system in vitro and in vivo

Background Chitosans of high molecular weights have emerged as efficient. nonviral gene delivery systems, but the properties and efficiency of well-defined low molecular weight chitosans ((.) 5 kDa) have not been studied. We therefore characterized DNA complexes Of Such low molecular weight chitosans and related their physical shape and stability to their efficiency as gene delivery systems in vit

Detection of neutron clusters

A new approach to the production and detection of bound neutron clusters is presented. The technique is based on the breakup of beams of very neutron-rich nuclei and the subsequent detection of the recoiling proton in a liquid scintillator. The method has been tested in the breakup of intermediate energy (30–50 MeV/nucleon) 11Li, 14Be, and 15B beams. Some six events were observed that exhibit the

Hydrophobic ligand binding properties of the human lipocalin apolipoprotein M

Apolipoprotein M (apoM) is a plasma protein associated mainly with HDL. ApoM is suggested to be important for the formation of pre beta-HDL, but its mechanism of action is unknown. Homology modeling has suggested apoM to be a lipocalin. Lipocalins share a structurally conserved beta-barrel, which in many lipocalins bind hydrophobic ligands. The aim of this study was to test the ability of apoM to