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Challenging Packaging Limits and Infectivity of Phage λ

The terminase motors of bacteriophages have been shown to be among the strongest active machines in the biomolecular world, being able to package several tens of kilobase pairs of viral genome into a capsid within minutes. Yet, these motors are hindered at the end of the packaging process by the progressive buildup of a force-resisting packaging associated with already packaged DNA. In this experi

Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device.

Live-cell imaging of biological processes at the single cell level has been instrumental to our current understanding of the subcellular organization of bacterial cells. However, the application of time-lapse microscopy to study the cell biological processes underpinning development in the sporulating filamentous bacteria Streptomyces has been hampered by technical difficulties. Here we present a

Cultural Trauma, Ritual and Healing

In this chapter I will discuss the effects of agency loss, cultural trauma and healing, by focusing on the impact the boarding schools, the so called residential school, had and still has on contemporary Cana-dian Mi’kmaq Native identity, drawing on interviews from one of my fieldworks conducted on the island Cape Breton, Nova Scotia.

Balancing Different Environmental Effects of Forest Residue Recovery in Sweden - A Stepwise Handling Procedure

An increased use of forest fuels is important in the reduction of our dependence on fossil fuels and in attempting to mitigate climate change. However, an increased use will also result in an intensified forestry. Recovery of forest residues (logging residues and stumps) results in a higher pressure on the forest ecosystems and adds environmental effects to those already existing in conventional f

The proper way of dwelling at the Early Neolithic gathering site of Almhov in Scania, Sweden

The Early Neolithic (c. 4000–3500 BC) site of Almhov, located in southwestern Scania, Sweden, is interpreted as a gathering and feasting site, subsequently transformed into a burial site with ancestral monuments. The focus of the article is on the pit pairs and pit clusters at the site, and on the dierential distribution of artefacts and animal bones within them, thereby touching upon more general

A Study on Serum Biochemistry and Hematological Profiling of Blue Rock Pigeon (Columba livia) in Multan (Punjab, Pakistan)

Thirty wild blue rock pigeons (Columba livia) were caught from Multan city (Pakistan). Hematological values were established for total red blood cells, total white blood cells, packed cell volume, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and differential leukocyte count. Serum bio chemistry values were determined for

Retinal Function after Vitrectomy

PURPOSE:: To study retinal function after vitrectomy. METHODS:: Core vitrectomy was performed in 12 rabbits under standardized conditions using a vitreous cutting rate of either 600 or 1200 cuts/min. Full-field electroretinography (ERG) and multifocal electroretinography (mfERG) were performed pre- and postoperatively. Morphologic change was monitored by immunohistochemistry directed against glial

Nutrient constraints on metabolism affect the temperature regulation of aquatic bacterial growth efficiency

Inorganic nutrient availability and temperature are recognized as major regulators of organic carbon processing by aquatic bacteria, but little is known about how these two factors interact to control bacterial metabolic processes. We manipulated the temperature of boreal humic stream water samples within 0-25 degrees C and measured bacterial production (BP) and respiration (BR) with and without i

Pregnancy and leisure time physical activity

ABSTRACT Aims: To examine associations between leisure time physical activity before and during pregnancy and preterm delivery, birth weight, and pre-eclampsia and to describe levels of physical activity prior to and during pregnancy. Furthermore to elucidate women’s experiences and views of leisure time physical activity during pregnancy in a qualitative study. Methods: Data sources were: the Aar

Gestational Diabetes Mellitus- Future risk for mother and child

Popular Abstract in Swedish Graviditetsdiabetes är diabetes som uppkommer eller först upptäcks under en graviditet. Mellan 1-14 % av alla gravida kvinnor runt om i världen drabbas av graviditetsdiabetes och i Sverige är det ungefär 2 %. I Sverige behandlas graviditetsdiabetes med kostråd och om detta inte räcker lägger man till insulinbehandling. Kända riskfaktorer för att utveckla graviditetsdiabGestational diabetes mellitus (GDM) occurs as a complication in 2% of all pregnancies in Sweden. Women with GDM have a substantial risk of developing type 2 diabetes later in life, but the risk of developing type 1 diabetes is also increased. GDM increases the risk for macrosomia and caesarean delivery. However, long term prognosis and eventual future risks for children born to mothers with a prev

Collapsed tetragonal phase in Ca(Fe1-xCox)(2)As-2 stabilized by pressure: Structural studies using single-crystal neutron diffraction

Ca(Fe1-xCox)(2)As-2 single crystals with concentration x = 0.032, 0.051, and 0.063 were investigated by means of neutron diffraction under hydrostatic pressure. The region of existence of the high-temperature tetragonal, low-temperature orthorhombic, and pressure-induced collapsed tetragonal phases have been determined and the corresponding p-T phase diagrams for the three compositions have been c

NMR diffusometry applied to liquids

Gradient based nuclear magnetic resonance spectroscopy provides a convenient method to determine self-diffusion coefficients especially in liquid samples. The method has many advantages including short experiment time, sensitivity, molecular resolution, and accuracy - all without the need for adding perturbing probes or chemically introducing isotopes. In this contribution we introduce the method

Tactical Driving Behavior With Different Levels of Automation

This paper investigated how different types of automation affect tactical driving behavior, depending on trust in the system. Previous research indicates that drivers wait for automation to act, delegating the monitoring of traffic situations. This would be especially true for those who have more trust in automation. Behavioral and gaze data from 30 participants driving an advanced simulator were

Excited state potential energy surfaces of bistridentate Ru-II complexes - A TD-DFT study

Time-dependent density functional theory (TD-DFT) calculations have been used to investigate low-energy singlet and triplet excited state potential energy surfaces (PES) of two prototype Ru-II-bistridentate complexes: [Ru-II(tpy)(2)](2+) (tpy is 2,2':6',2 ''-terpyridine) and [Ru-II(dqp)(2)](2+) (dqp is 2,6-di(quinolin-8-yl)pyridine). Solvent effects were considered using a self-consistent reaction

Subjective Sleep Complaints Are Associated With Insulin Resistance in Individuals Without Diabetes The PPP-Botnia Study

OBJECTIVE-Sleep disorders and subjective sleep complaints have been associated with increased risk of type 2 diabetes. The evidence with respect to insulin resistance (IR) and insulin secretion in individuals without type 2 diabetes has been scarce and elusive. We examined if subjective sleep complaints and their co-occurrence were associated with IR and insulin secretion in adult women and men wi

Motives for sports participation as predictions of self-reported outcomes after anterior cruciate ligament injury of the knee

Aim of the study was to access how individual's motives for participation in sports impact on self-reported outcomes 2 years after an anterior cruciate ligament injury. Based on a longitudinal cohort study, this secondary analysis present data from the Knee Anterior Cruciate Ligament, Nonsurgical versus Surgical Treatment (KANON) study, a randomized controlled trial. At baseline, 121 patients reco