Construction of a Novel miRNA Library Preparation kit for Next Generation Sequencing
MicroRNAs (miRNAs) är små, enkelsträngade RNA molekyler som består av cirka 22 molekylära byggstenar, nukleotider. Nukleotiderna består antingen av adenin (A), guanin (G), cytosin (C) eller uracil (U). Dessa små RNA molekyler är involverade i flertalet sjukdomar och genom att hitta sekvensen av dessa A, G, C, T i ett biologiskt prov, kan miRNAt agera som markör för att hitta nya behandlingsmetoderMicroRNAs are small single stranded RNA molecules containing approximately 22 nucleotides and these play an important regulatory role in gene expression. MicroRNAs are found to be involved in several diseases and knowledge about their sequences can be an important tool in the foundation of novel treatment methods against diseases. Today, these small RNA molecules are mainly analyzed with qPCR and
