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Popular Abstract in Swedish Fältflödesfraktionering-ett verktyg för att optimera genteknisk produktion av proteiner Läkemedel av proteintyp kan i framtiden tillverkas mer kostnadseffektivt. Utbytet av protein maximeras genom att den proteinproducerande bakteriens ribosomer separeras och räknas. Proteiner i vår vardag Protein kan vara mer än den blodiga biffen eller torskfilén på lunchtallriken. PIn this thesis asymmetrical flow field-flow fractionation (FFF) separations of ribosomes and subunits have been studied. Ribosomal material was prepared from Escherichia coli (E. coli) cells and analyzed by the size separation technique. The five main peaks in the fractograms correspond to transfer RNA (tRNA), unidentified proteins, small subunit, large subunit, and ribosome. The accuracy of the

Purpose: To search for the tube voltage which results in the highest clinical image quality per effective dose unit for chest and pelvis radiography respectively, using image plates. Methods: Two anthropomorphic phantoms were imaged with several different tube voltages. For the chest phantom, the tube voltage was varied between 70 and 150 kV, and for the pelvis phantom between 50 and 102 kV. The m

Capacitive biosensors were developed for several analytes such as hormones, interleukines, viruses and heavy metal ions. The sensing surfaces were prepared by immobilizing the biorecognition element to self-assembled monolayers (SAMs) on gold. SAMs form spontaneously on gold by adsorption of thiols, sulfides or disulfides. After immobilization of the biorecognition element a long chain hydrocarbon

To assess the distribution of blood from the umbilical vein (UMV), inferior vena cava (IVC) and superior vena cava (SVC) to either side of the fetal lamb heart by using ultrasound enhanced contrast agent imaging. By injection of ultrasound contrast agents (UCA) in UMV, IVC or SVC, the blood from these vessels was tracked, as it then was highly echogenic. By evaluating the image intensity within th

The Great Rift Valley running through Eastern Africa contains a large number of soda lakes. These unique lakes constitute among the most stable alkaline environments with a dense and varied population of microorganisms. Such microorganisms are invariable alkaliphiles and are considered to be a promising source of enzymes with unique features. Some enzymes produced by alkaliphilic microorganisms ha

Ultrafast optical response and excitation energy dynamics in light harvesting pigment-protein complexes and in a few model systems have been studied both experimentally and theoretically. Femtosecond light pulses were employed to excite the system and monitor the subsequent time evolution of the transient absorption kinetics and anisotropy. A new form of pump-probe technique was developed for perf