Competition between transglycosylation and hydrolysis in almond beta-glucosidase-catalyzed conversion of p-nitrophenyl-beta-D-glucoside in monophasic water/alcohol mixtures
Almond beta-glucosidase was used to catalyze the transglycosylation of p-nitrophenyl-beta-D-glucoside to alkyl glucosides, with hydrolysis to glucose as a side reaction. The conversions were carried out in alcohols with varying water contents below water saturation. Both the total reaction rate and the ratio between the transglycosylation and hydrolysis increased with increasing water activity, an
