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Cloning, sequence analysis, and expression of a gene encoding an endoxylanase from Bacillus halodurans S7

The gene encoding an alkaline active xylanase of Bacillus halodurans S7, containing an open reading frame of 1188 nucleotides encoding 396 amino acids, was cloned and expressed in Escherchia coli. On the basis of sequence similarity, possible -10 and -35, ribosome binding, and transcription terminator regions were identified. Analysis of the deduced amino acid sequence revealed that the protein wa

Polybrominated diphenyl ethers at a solid waste incineration plant II: atmospheric deposition

In the second of two papers, the atmospheric polybrominated diphenyl ethers (PBDE) deposition (wet and dry) at a municipal solid waste incineration (MSW) plant with electronics recycling is compared with that at an industrial urban reference site (URS) producing asphalt and concrete. Results of BDE209 and SigmaPBDE excluding BDE209 as representatives of "new" vs. "old" PBDEs are presented. Volume

Mutations in the endosomal ESCRTIII-complex subunit CHMP2B in frontotemporal dementia

We have previously reported a large Danish pedigree with autosomal dominant frontotemporal dementia (FTD) linked to chromosome 3 (FTD3). Here we identify a mutation in CHMP2B, encoding a component of the endosomal ESCRTIII complex, and show that it results in aberrant mRNA splicing in tissue samples from affected members of this family. We also describe an additional missense mutation in an unrela

Outbildad personal i hemtjänsten utsätter vårdtagaren för risk. Bättre kunskaper i läkemedelshantering måste krävas

During the last few years the home care services have had to face an increased responsibility for medical tasks. A study in the city of Malmö in 1993, repeated in 1998, shows that 95% of the health care assistants are engaged in the administration of medication. The studies demonstrate that health care assistants have inadequate knowledge not only about medicines and their administration but also

Local cerebral blood flow in the rat during severe hypoglycemia, and in the recovery period following glucose injection

In order to assess the influence of severe hypoglycemia on local cerebral blood flow (1-CBF) artificially ventilated rats, maintained on 70% N2O, were injected with insulin to provide either an EEG pattern of slow-wave polyspikes, or cessation of spontaneous EEG activity for 5, 15 or 30 min ("coma"). In other animals, glucose was injected at the end of a 30 min period of "coma" and 1-CBF was measu

Mechanical response of wood perpendicular to grain when subjected to changes of humidity

This paper describes the deformations caused by stress and humidity interaction, mechano-sorption, in the cross grain directions of wood and the relaxation or accumulation of internal stresses caused by these deformations. Long-term tests on small clear specimens in cyclic climates with both tensile and compressive loads were carried out. The development of internal stresses in timber was measured

On acoustic particle and cell manipulation in microfluidic systems

Popular Abstract in Swedish En kombination av laminära flöden i mikrofabricerade kanaler och akustiska krafter genererade i stående ultraljudsvågor erbjuder nya möjligheter för avancerad partikel- och cellhantering i både lab-on-a-chip-system och applikationer med högre kapacitetskrav. Akustisk partikelhantering kan användas exempelvis för att separera, tvätta, sortera, låsa fast och distribuera pThe combination of laminar flows in microfabricated channels and acoustic forces induced in ultrasonic standing wave fields offers new possibilities for advanced particle and cell manipulation in lab-on-a-chip applications as well as in relatively high throughput applications. Acoustic particle manipulation systems can be used e.g. to separate, wash, sort, trap or distribute particles in microflui

Complement analysis in the 21st century

Complement analysis in the clinic is usually associated with the quantification of C3 and C4, measurement of C1-inhibitor and screening for complement activity. These analyses have been available in routine diagnostic laboratories for decades. In recent years, however, the field of complement analysis has expanded considerably, with the introduction of novel assays to detect complement activation

Survival of patients with kyphoscoliosis receiving mechanical ventilation or oxygen at home

Background: Home mechanical ventilation (HMV) and long-term oxygen therapy (LTOT) are the two treatment alternatives when treating respiratory insufficiency in patients with kyphoscoliosis. We aimed to study the effect on survival with regard to HMV or LTOT alone in patients with respiratory insufficiency due to kyphoscoliosis. Methods: Swedish patients with nonparalytic kyphoscoliosis (ie, scolio

A catalytic oxidation of sugar by vanadium(IV)

Platinum and other nobel metals have been found to catalyze the complete oxidation of sucrose and other sugars by vanadium(IV) in acid environments. As the redox potential of vanadium(IV)/vanadium(III) differs substantially from that of dioxygen/water, it might be possible to use this reaction to construct a redox type of fuel cell. In the present paper, the requirements on the catalyst are discus

Inconsistency between glycemic and insulinemic responses to regular and fermented milk products

Background: Foods with a low glycemic index are increasingly being acknowledged as beneficial in relation to the insulin resistance syndrome. Certain organic acids can lower the glycemic index of bread products. However, the possible effect of acids in fermented milk products on the glycemic index and on insulinemic characteristics has not been addressed. The metabolic effects of fermented milk or

Influence of sympathetic stimulation on the mechanical properties of the aorta in humans

The mechanical properties of the aorta play a major role in the regulation of blood pressure and cardiac performance. The effect of sympathetic stimulation on the mechanical properties of the human abdominal aorta was studied in 19 healthy volunteers, divided into young (25 +/- 2 years) and elderly individuals (69 +/- 2 years) of both sexes. A non-invasive ultrasonic echo-tracking system for measu