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The contribution of nonspecific interactions to the overall interactions of thiol-ssDNA and dsDNA macromolecules with gold nanoparticles was investigated. A systematic investigation utilizing dynamic light scattering and cryogenic transmission electron microscopy has been per-formed to directly measure and visualize the changes in particle size and appearance during functionalization of gold nanop
Selective isolation of lactate dehydrogenase (LDH) from porcine muscle extract was studied using foam generated from the vigorous stirring of a non-ionic surfactant, Triton X-114 derivatized with Cibacron blue. The cloud point of the surfactant-dye conjugate was higher than that of the native Triton X-114, and also the foam prepared from the affinity surfactant was more rigid taking a longer time
This article examines the recent development of, and policy rationale for research pooling initiatives in Scotland. Research pooling initiatives have been started with financial support from the Scottish Funding Council in order to form inter-organizational international critical mass of research excellence at universities at the regional level. This article examines the development of university
Poor survival and differentiation of grafted dopamine neurons limits the application of clinical transplantation in Parkinson’s disease. The survival of grafted dopamine neurons is only improved by a factor of 2–3 by adding neuroprotectants during tissue preparation. We used dye exclusion cell viability and electron microscopy to investigate the effects of the caspase inhibitor ac-YVAD-cmk and the
Background: Hemophilia A patients with inhibitors are generally treated with preparations containing activated coagulation factors to achieve hemostasis by bypassing factor (F)VIII. Objectives: We developed an assay for monitoring the kinetic of thrombin generation in human FVIII inhibitor plasma reconstituted in vitro with activated prothrombin complex concentrate, FEIBA, and in plasma samples fr
Laminins are important for basement membrane structure and function. The laminin alpha2 chain is a major component of muscle basement membranes, and mutations in the laminin alpha2 gene lead to congenital muscular dystrophy in humans and mice. Although the laminin alpha2 chain is prominently expressed in testicular basement membranes, its role in testis has remained unclear. Here, we show that lam
The aim of this paper is to investigate whether or not ethical conflicts can be identified, analysed and solved using ethical principles. The relation between the physician and the patient with ischemic heart disease (IHD) as life style changes are recommended in a secondary prevention program is used as an example. The principal persons affected (the patient and his or her spouse) and the ethical
In the mobile industry, system platforms are offered to device developers to enable rapid product development while sharing expensive technology development investments. This paper presents a framework for assessment of requirements engineering collaboration related to statements-of-compliance negotiation in platform subcontracting. The framework includes a classification of platform compliance sc
We performed kinematic studies of the evolution of small-scale magneticfields in the surface layers of M-dwarfs. We solved the inductionequation for a prescribed velocity field, magnetic Reynolds number ReM,and boundary conditions in a Cartesian box, representing a volumecomprising the optically thin stellar atmosphere and the uppermost partof the optically thick convective envelope. The velocity
The failure of many fisheries world-wide, and the concern about marine biodiversity, has sparked a growing interest in the spatial aspects of harvested populations. If a population conforms to the Ideal Free Distribution and that one of the habitats is set aside as a reserve free from harvesting, the design of reserves may be problematic. If a substantial proportion of the unharvested population i
Using coupled cluster singles and doubles linear response theory and the d-aug-cc-pVTZ basis set extended with a 3s3p2d1f1g set of midbond functions, the interaction induced electric dipole polarisability surface of the CO-Ar van der Waals complex is computed. Combining this surface with accurate intermolecular potential energy and electric dipole surfaces, the pressure and dielectric second viria
Energy crops (willow, sugar beet and grass silage) were digested ill pilot scale two-stage anaerobic digesters. The specific methane yields obtained were 0.16, 0.38 and 0.39 m(3) kg(-1) added volatile solids (VSadded) for willow, sugar beet and grass, respectively, corresponding to yearly gross energy yields of 15, 53 and 26 megawatt-hours (MWh) per hectare. With grass and sugar beets as substrate
The in vitro digestion of a self nano-emulsifying drug delivery system (SNEDDS) was visualized by cryogenic transmission electron microscopy (Cryo-TEM). The dynamic lipolysis model, simulating the environment of the gastrointestinal tract in fasted conditions, was used for this purpose. The results revealed that micelles are present during the entire lipolysis process. Oil droplets from the self n
We demonstrate how rather hydrophilic surfactants can be used for solubilizing simultaneously water and alkane. The required hydrophilic-lipophilic balance can be achieved by the addition of a medium-chain alcohol, that is, a hydrophobic cosurfactant. Specifically, the phase behavior of the quaternary water-n-octane-n-octyl beta-glucopyranoside (C(8)G(1))-n-octanol (C8E0) system has been investiga
In this study, typical load shapes for two categories of Swedish commercial and public buildings – schools and hotels – are presented and discussed. The measurements from 13 schools and 9 hotels in the south part of Sweden were analysed. Load shapes are developed for different mean daily outdoor temperatures and different day-types - standard weekdays and standard weekends. The load shapes are pre
Auditory event-related brain potentials evoked in response to tone stimuli and to speech stimuli were recorded in a group of parents of children with specific language-impairment and a group of parents of normally speaking children. The parents of the language-impaired children showed longer P3 latencies than the parental control group in the speech task requiring a phonological discrimination, bu
