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Tamoxifen therapy resistance constitutes a major cause of death in patients with recurrent estrogen receptor (ER) positive breast cancer. Through high resolution mass spectrometry (MS), we previously generated a 4-protein predictive signature for tamoxifen therapy outcome in recurrent breast cancer. ANXA1 and CALD1, which were not included in the classifier, were however the most differentially ex

We recently reported on the development of a 4-protein-based classifier (PDCD4, CGN, G3BP2, and OCIAD1) capable of predicting outcome to tamoxifen treatment in recurrent, estrogen-receptor-positive breast cancer based on high-resolution MS data. A precise and high-throughput assay to measure these proteins in a multiplexed, targeted fashion would be favorable to measure large numbers of patient sa

Laser-capture microdissection (LCM) offers a reliable cell population enrichment tool and has been successfully coupled to MS analysis. Despite this, most proteomic studies employ whole tissue lysate (WTL) analysis in the discovery of disease biomarkers and in profiling analyses. Furthermore, the influence of tissue heterogeneity in WTL analysis, nor its impact in biomarker discovery studies have

Estrogen receptor (ER)-positive breast cancer represents the majority (∼70%) of all breast malignancies. In this subgroup of breast cancers, endocrine therapies are effective both in the adjuvant and recurrent settings, although resistance remains a major issue. Several high-throughput approaches have been used to elucidate mechanisms of resistance and to derive potential predictive markers or alt

We have previously identified UMP-CMP kinase (CMPK1) as a prognostic marker for triple negative breast cancer (TNBC) by mass spectrometry (MS). In this study we evaluated CMPK1 association to prognosis in an independent set of samples by immunohistochemistry (IHC) and assessed biological pathways associated to its expression through gene set enrichment analysis (GSEA). A total of 461 TNBC paraffin

In a previous study, we detected a significant association between phosphoserine aminotransferase 1 (PSAT1) hyper-methylation and mRNA levels to outcome to tamoxifen treatment in recurrent disease. We here aimed to study the association of PSAT1 protein levels to outcome upon tamoxifen treatment and to obtain more insight in its role in tamoxifen resistance. A cohort of ER positive, hormonal thera

Decentralisation in Cambodia has long been propagated as a means to enhance local engagement with governance structures. But in the forestry sector, even limited devolution of powers often constrains local user groups with excessive bureaucratic burdens. In addition, entrenched political economy interests tend to inhibit effective governance. To investigate the apparent institutional malaise that

Many policy targets have been developed with the aim of reaching a more sustainable development; more specifically, thestudied policies target energy use and urban growth. For example, by 2050 the aim is that the European Union will havereduced its greenhouse emissions by 90%. Examples of policy targets regarding urban growth can be found in many ofthe larger cities, as they have developed plans fMany policy targets have been developed with the aim of reaching a more sustainable development; more specifically, thestudied policies target energy use and urban growth. For example, by 2050 the aim is that the European Union will havereduced its greenhouse emissions by 90%. Examples of policy targets regarding urban growth can be found in many ofthe larger cities, as they have developed plans f

BACKGROUND: Societal interest in enabling older workers to prolong their working life has increased in recent years due to demographic change. Managers have a key role in extending working life among employees, because they control the organisation, work environment and potential measures enabling older workers to extend their working life. OBJECTIVE: To examine managers' attitudes to their older

An increasing number of older people in the population will bring new challenges for the society and care coordination. One of the most important questions in care coordination is the employees’ work performance. The overall aim of this study was to examine care employees’ experience of factors that rule how they allocate their time and tasks in the care work. The study was qualitative and consist

We report on the study of six similar buildings built in an area of Gothenburg, Sweden,in 1971, which are now in urgent need of renovation. However, the owner of thebuildings - a municipal housing company did not achieve a financially viablerenovation of the pilot project. This meant that renovation on a similar basis for theremaining five buildings would not be possible. For this reason the housi

The concept of video- or computergame addiction has entered the popular vocabulary as a common way of talking about the conflicts and troubles emerging in relation to video gameplay in the socio-cultural contexts of everyday life. Whether they appear in newspaper articles announcing the advent of a new grave diagnosis, or in the arguments between teenagers and their adults with regard to the prope

Abundant data is the key to successful machine learning. However, supervised learning requires annotated data that are often hard to obtain. In a classification task with limited resources, Active Learning (AL) promises to guide annotators to examples that bring the most value for a classifier. AL can be successfully combined with self-training, i.e., extending a training set with the unlabelled e

Sialic acids comprise a varied group of nine-carbon amino sugars that are widely distributed among mammals and higher metazoans. Some human commensals and bacterial pathogens can scavenge sialic acids from their environment and degrade them for use as a carbon and nitrogen source. The enzyme N-acetylmannosamine kinase (NanK; EC 2.7.1.60) belongs to the transcriptional repressors, uncharacterized o