Helicobacter species are fastidious bacterial pathogens that are difficult to culture by standard methods. A PCR-denaturing gradient gel electrophoresis (PCR-DGGE) technique for detection and identification of different Helicobacter species was developed and evaluated. The method involves PCR detection of Helicobacter DNA by genus-specific primers that target 16S rDNA and subsequent differentiatio

The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Satellite II-rich 1qh and 16qh display high frequencies of abnormalities in mitogen-

Hans Rott has argued, most recently in his book Change, Choice and Inference, that certain formal correspondences between belief revision and rational choice have important philosophical implications, claiming that the former strongly indicate the unity of practical and theoretical reason as well as the primacy of practical reason. In this paper, I confront Rott's argument with three serious chall

In this short note, some experimental results are presented on the diffraction of a spherical way by a hard half-plane. This study was conducted with the aim to give evidence to the existence of the edge-diffracted wave. The sound source used in this experimental study is a condenser microphone operating in a reverse way. The wave emitted by a sound source propagates in space and hits a thin alumi

A newly isolated, purified, and characterized PQQ-dependent alcohol dehydrogenase (a bacterial membrane-bound protein) was recently found to display a surprisingly large linear range and high selectivity towards ethanol when integrated into a conducting polymer network on a platinum electrode. These findings motivated us to study the enzyme when simply immobilized onto carbonaceous surfaces in ord

Biological pathways represent the relationships (reactions and interactions) between biological molecules in the context of normal cellular functions and disease mechanisms. Understanding the roles of proteins and signaling pathways expressed within disease, and their link to drug discovery and drug development are central in today's target-driven pharmaceutical processes. This article gives an ov

The idea of dynamic programming is general and very simple, but the "curse of dimensionality" is often prohibitive and restricts the fields of application. This paper introduces a method to reduce the complexity by relaxing the demand for optimality. The distance from optimality is kept within prespecified bounds and the size of the bounds determines the computational complexity. Several computati

Many vehicle manufacturers have lately introduced advance driver support in some of their automobiles. One of those new features is Adaptive Cruise Control DACCE, which extends the conventional cruise control system to control of relative speed and distance to other vehicles. In order to design an ACC controller it is suitable to have a model of driver behavior. The approach in the thesis is to us

Two-components cycle-resolved Laser Doppler Velocimetry (LDV) measurements were performed with high data rate to resolve the flow in a spark ignition engine. The velocity measurements were then processed with both a frequency and a time domain filtering technique to obtain the level of turbulence at different frequencies. Simultaneous cylinder pressure measurements gave information of the duration

The configuration-dependent cranked Nilsson-Strutinsky model is used to study the band structures in La-131. Shape coexistence and band termination are investigated for several configurations. The calculations indicate that La-131 is a gamma-soft nucleus. The theoretical results are compared with experimental data. The calculated pi( h(11/2))(2) circle times nu( h(11/2))(8) configuration with (pi,

A new nuclear microscopy technique called ion photon emission microscopy or IPEM was recently invented. IPEM allows analysis involving single ions, such as ion beam induced charge (IBIC) or single event upset (SEU) imaging using a slightly modified optical microscope. The spatial resolution of IPEM is currently limited to more than 10 mu m by the scattering and reflection of ion-induced photons, i

The benefits of increased parental participation in the care of the hospitalised child is well documented, and care given by parents is found to be effective. To facilitate the child’s recovery by providing the parents with information and education about care, and to minimise disruption to the child’s and the family’s normal life the care after hypospadiasis repair was changed. Outcomes were eval

n instrument suitable for measuring the total water content both as small liquid droplets and as vapour in the air, in water mist total flooding extinguishing tests, is presented. The instrument consists of a heater and a Vaisala humidity meter. The Vaisala humidity meter is capable of measuring relative humidity at temperatures up to 180°C. The instrument has a measuring range of 0-600g/m3, a sam