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Coupled biosensor, biomimetic and chemometrics strategies for analysis of the metals in complex environmental matrices

An analytical strategy to determine metals in environmental samples contaminated with both organophosphate pesticides and metals is presented. In the first step, parathion and arsenate were determined separately. Inhibition of the enzyme butyrylcholinesterase was quantified employing an enzyme thermistor and also with a CCD camera. Subsequently, parathion mixed with arsenate was measured. A molecu

Galanin expression in sensory neurons after nerve compression or transection.

Galanin is probably involved in nociceptive sensory processing in spinal cord. We investigated whether a common injury, peripheral nerve compression, induced up-regulation of galanin (immunocytochemistry) in sensory neurons in rats 6 or 14 days post-injury and compared the response with other nerve injuries. Sciatic nerve compression increased the number of galanin positive sensory neurons as comp

Diffusion-weighted MRI findings in patients with capsular warning syndrome

Background and Purpose: The 'capsular warning syndrome' (CWS) of recurrent stereotyped episodes of motor or sensory dysfunction is clinically well recognized, and is associated with a high risk of imminent lacunar infarction with permanent deficits resembling those of CWS. However, the pathophysiology of CWS has not been well characterized. We report a clinicoanatomic correlation with MR imaging s

Synchronized Quartz Crystal Microbalance and Nanoplasmonic Sensing of Biomolecular Recognition Reactions

We present a method providing synchronized measurements using the two techniques: quartz crystal microbalance with dissipation (QCM-D) monitoring and localized surface plasmon resonance (LSPR). This was achieved by letting a thin gold film perforated with short-ranged ordered plasmon-active nanoholes act as one of the electrodes of a QCM-D crystal. This enabled transmission-mode optical spectrosco

The effect of intestinal ischemia and reperfusion injury on ICAM-1 expression, endothelial barrier function, neutrophil tissue influx, and protease inhibitor levels in rats.

Multiple organ dysfunction syndrome (MODS) is mediated by complex mechanisms in which interactions between activated leukocytes and endothelial cells play a central role. ICAM-1 (intercellular adhesion molecule-1) mediates firm adhesion and transendothelial migration of activated leukocytes from postcapillary venules into the tissue. The present study evaluated the ICAM-1 expression in various org

Physical exercise, sports, and lung function in smoking versus nonsmoking adolescents

Associations between adolescent smoking habits and exercise, particularly participation in sports and lung function were studied. All students aged 13-19 yrs in Nord-Trondelag County, Norway, 1995-1997, were invited to join a cross-sectional study. Information on smoking habits and exercise was obtained by self-administered questionnaire. Spirometry was performed in accordance with American Thorac

The molecular pathogenesis of collagen-induced arthritis in mice--a model for rheumatoid arthritis.

The most widely used model for rheumatoid arthritis is the collagen-induced arthritis (CIA) in mice. This model has gained acceptance since it is reproducible, well defined and has proven useful for development of new therapies for rheumatoid arthritis, as exemplified by the most recent advancement using TNFalpha neutralization treatment. The collagen-induced arthritis model, however, represents o

Harmonic microchip acoustophoresis: a route to online raw milk sample precondition in protein and lipid content quality control

A microfluidic approach for raw milk sample preconditioning prior to protein and lipid content analysis has been developed. The system utilizes microchip acoustophoresis and is a further extension of our previously reported multiple node ultrasonic standing wave focusing platform ( Grenvall , C. , Augustsson , P. , Matsuoka , H. and Laurell , T. Proc. Micro Total Anal. Syst. 2008 , 1, 161-163 ). T

Impact of polymorphisms of the major histocompatibility complex class II, interleukin-10, tumor necrosis factor-alpha and cytotoxic T-lymphocyte antigen-4 genes on inhibitor development in severe hemophilia A

Background: Approximately 25% of severe hemophilia A (HA) patients develop antibodies to factor VIII protein. Patients: In the present case-controlled cohort study, 260 severely affected, mutation-type-matched HA patients were studied for association of human leukocyte antigen (HLA) class II molecules and polymorphisms in the genes encoding interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-

Tracking carbon from the atmosphere to the rhizosphere

Turnover rates of arbuscular mycorrhizal (AM) fungi may influence storage of soil organic carbon (SOC). We examined the longevity of AM hyphae in monoxenic cultures; and we also used C-13 incorporation into signature fatty acids to study C dynamics in a mycorrhizal symbiosis involving Glomus intraradices and Plantago lanceolata. C-13 enrichment of signature fatty acids showed rapid transfer of pla

Articular, monoclonal gamma3 heavy-chain deposition disease: characterization of a partially deleted heavy-chain gene and its protein product synthesized in vivo and in vitro.

Objective A patient presented with heavy-chain deposition disease (HCDD), exhibiting severe erosive polyarthropathy caused by synovial deposits of abnormal monoclonal, heavily deleted free 3 heavy chains lacking the VH and CH1 domains. The absence of VH was surprising, since it is considered important for pathogenic tissue deposition. This study was undertaken to analyze the genetic structure of

A microcarrier cell culture system for large scale production of hepatitis A virus

Hepatitis A virus (HAV) was isolated from human faeces using a fetal rhesus monkey kidney cell line (Frhk-4). Infectious medium from passage 12 was used to inoculate a large (5000 cm2) microcarrier cell culture maintained in suspension. The microcarriers used were swollen, collagen-coated dextran beads on which it was easy to propagate Frhk-4 cells. Intra- and extra-cellular virus levels were assa

Density-dependent competition and selection on immune function in genetic lizard morphs

Density-dependent territorial interactions have been suggested to cause immunosuppression and thereby decrease fitness, but empirical support from natural populations is lacking. Data from a natural lizard population (Uta stansburiana) showed that breeding females surrounded by many territorial neighbors had suppressed immune function. Furthermore, variation in immunological condition had differen

A chemosorptive cylindrical denuder designed for personal exposure measurements of isocyanates-evaluation on generated aerosols of 4,4 '-methylenediphenyl diisocyanate

A denuder/filter system constructed for solvent-free personal exposure measurements was evaluated for separation of vapour and particulate 4,4'-methylenediphenyl diisocyanate (4,4'-MDI) generated from heated PUR-foam. The two different phases were collected in the denuder and on the filter, respectively, by chemosorption on a polydimethylsiloxane (SE-30)-dibutylamine (DBA) stationary phase. Both r

Impact of sewage sludge application on the long-term nutrient balance in acid soils of Scots pine (Pinus sylvestris, L.) forests

The objective of the investigation was to determine the effects of sewage sludge application on nutrient concentrations in soil and plant biomass fractions in Scots pine forests (Pinus sylvestris, L.), situated on sandy soils with low pH, in a south to north temperature gradient in Sweden. Twenty tons dw ha(-)1 of sewage sludge was applied in 50 to 60 yr old pine forests at four sites from Brosarp

H-1 NMR diffusometry study of water in casein dispersions and gels

The self-diffusion coefficients of water in casein solutions and gels were measured using a pulsed-gradient spin-echo nuclear magnetic resonance technique (PGSE NMR). The dependence of the self-diffusion coefficient of water on the concentration and structure-of casein is reported. The results were analyzed using a cell model. It was found that the water self-diffusion coefficient is insensitive t

Spectroscopic observations, spin-orbit functions, and coupled-channel deperturbation analysis of data on the A (1)Sigma(+)(u) and b (3)Pi(u) states of Rb-2

We present experimentally derived potential curves and spin-orbit interaction functions for the strongly perturbed A (1)Sigma(+)(u) and b (3)Pi(u) states of the rubidium dimer. The results are based on laser-induced fluorescence and optical-optical double-resonance polarization spectroscopy measurements combined with earlier laser-induced fluorescence data. We used an analytic potential (Hannover