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Identification of SLPI (Secretory leukocyte protease inhibitor) in human mast cells using immunohistochemistry and in situ hybridisation

Recently interest has been focused on secretory leucocyte protease inhibitor (SLPI) and its role in immediate hypersensitive reactions, possibly by inhibiting mast cell chymase. The purpose of this investigation was to show whether or not SLPI is produced in mast cells. Double-immunolabelling revealed that SLPI coexists with mast cell tryptase (60%) and chymase (37%). On the other hand, in situ hy

Solvent Paths for Square-Planar Substitutions : Part 2. Reactions between Aqua Chloro-Platinates(II) and Ethene

The kinetics for the reactions between the complexes PtCln(H2O)4-n 2−n, n = 0,1,2,3,4, (including cis- and trans-isomers for n=2) and ethene have been studied. In the case of trans-PtCl2(H2O)2, product analysis shows that both the chloride ligand and the aqua ligand are substituted by ethene in two parallel paths, differing in rate by a factor of only 1.5. The experiments show that ethene, and proThe kinetics for the reactions between the complexes PtCln(H2O)4-n 2−n, n = 0,1,2,3,4, (including cis- and trans-isomers for n = 2) and ethene have been studied. In the case of trans-PtCl2(H2O)2, product analysis shows that both the chloride ligand and the aqua ligand are substituted by ethene in two parallel paths, differing in rate by a factor of 1.5 only. The experiments show that ethene, and p

IgE-mediated histamine release from nasal mucosa is inhibited by SLPI (secretory leukocyte protease inhibitor) to the level of spontaneous release

The secretory leukocyte protease inhibitor (SLPI) is a low-molecular- weight inhibitor of proteases, such as elastase and cathepsin G which are released from leukocytes during phagocytosis. The purpose of this study was to determine whether or not SLPI is able to inhibit IgE-mediated histamine release. Nasal mucosa from 11 test subjects without atopic disposition was used for this in vitro study.

Localisation of secretory leucocyte proteinase inhibitor mRNA in nasal mucosa

Human secretory leucocyte proteinase inhibitor (SLPI) is a low-molecular weight, acid-stable inhibitor of polymorphnuclear granulocyte elastase and cathepsin G. Previous reports have demonstrated the existence of SLPI in the respiratory tract, salivary glands and cervical mucosa. Positive staining for SLPI using immunohistochemical techniques has been reported in serous glands in nasal mucosa. We

The Solvent Path in Square-Planar Substitutions. Kinetics and Mechanism for Reactions of Tetrachloroplatinate(II) and Aquachloroplatinates(II) with Halides, Thiocyanate and Dimethyl Sulfoxide

The kinetics for the reactions between the six complexes PtCln(H2O)4-n−2n, n = 0,1,2,3,4 (including cis- and trans-isomers for n=2) and the entering ligands Y = Cl−, Br−, I−, SCN−, and DMSO have been studied. The experiments with PtCl42− and trans- PtCl2(H2O)2 give the usual two-term rate constant kexp = k1 + k2[Y], when no extra chloride has been added to the solutions. The parameter k1 can be id

Measured adiposity in relation to head and neck cancer risk in the European Prospective Investigation into Cancer and Nutrition

BACKGROUND: Emerging evidence from cohort studies indicates that adiposity is associated with greater incidence of head and neck cancer (HNC). However, most studies have used self-reported anthropometry which is prone to error.METHODS: Among 363 094 participants in the European Prospective Investigation into Cancer and Nutrition study (EPIC) with measured anthropometry, there were 837 incident cas

Both the Rule and the Exception : The Concept of a Legal Dilemma and the Survival of the State

The paper considers whether there can be legal states of affairs that are both the rule and the exception or, in other words, whether there can be situations where the rule and the exception are superimposed upon each other so that neither the rule nor the exception singularly controls the legal classification of a given situation although both the rule and the exception continue to apply. The papThe paper considers whether there can be legal states of affairs that are both the rule and the exception or, in other words, whether there can be situations where the rule and the exception are superimposed upon each other so that neither the rule nor the exception singularly controls the legal classification of a given situation although both the rule and the exception continue to apply. The pap

Cylindrical multipole expansion for periodic sources with applications for three-phase power cables

This paper presents a cylindrical multipole expansion for periodic sources with applications for three-phase power cables. It is the aim of the contribution to provide some analytical solutions and techniques that can be useful in the calculation of cable losses. Explicit analytical results are given for the fields generated by a three-phase helical current distribution and which can be computed e

N-Spirocyclic quaternary ammonium ionenes for anion-exchange membranes

The development of cationic polymers for anion-exchange membranes (AEMs) with high alkaline stability and conductivity is a considerable challenge in materials chemistry. In response, we here present the synthesis and properties of N-spirocyclic quaternary ammonium ionenes (spiro-ionenes) containing 5- and 6-membered rings fused by central nitrogen cations. High-molecular weight and film-forming s

Adsorption of polyelectrolyte-like proteins to silica surfaces and the impact of pH on the response to ionic strength. A Monte Carlo simulation and ellipsometry study

Hypothesis The adsorbed amount of the polyelectrolyte-like protein histatin 5 on a silica surface depends on the pH and the ionic strength of the solution. Interestingly, an increase in ionic strength affects the adsorbed amount differently depending on the pH of the solution, as shown by ellipsometry measurements (Hyltegren, 2016). We have tested the hypothesis that the same (qualitative) trends

Functional central limit theorems for the Nelson–Aalen and Kaplan–Meier estimators for dependent stationary data

We derive process limit distribution results for the Nelson–Aalen estimator of a hazard function and for the Kaplan–Meier estimator of a distribution function, under different dependence assumptions. The data are assumed to be right censored observations of a stationary time series. We treat weakly dependent as well as long range dependent data, and allow for qualitative differences in the depende

Modeling the electrostatic charging of a helicopter during hovering in dusty atmosphere

A helicopter flying through an atmosphere containing particulates may accumulate high electrostatic charges which can challenge its operational safety. In this paper we elaborate and validate a triboelectric charging model to predict the electrification experienced by a helicopter while hovering in dusty air. We employed Large Eddy simulations to describe the turbulent structures inherent in the f

Effects of Ca addition on the uptake, translocation, and distribution of Cd in Arabidopsis thaliana

Cadmium (Cd) pollution poses a risk to human health for its accumulation in soil and crops, but this can be alleviated by calcium (Ca) addition. However, its mechanism remains unclear yet. In this study, Arabidopsis thaliana was used to explore the alleviating effects of Ca on Cd toxicity and its specific function during uptake, upward-translocation, and distribution of Cd. Supplementing plants wi

A novel optimization framework for designing multi-stream compact heat exchangers and associated network

Despite the performance gains of heat exchangers by thermal-hydraulic optimization, many design approaches suffer from a long process of trial-and-error, limited practical problem formulation and poor robustness. This paper addresses those challenges and develops an optimization framework for designing multi-stream compact heat exchangers. The optimization framework consists of a multi-stream matc

Analysis of Agaricus meleagris pyranose dehydrogenase N-glycosylation sites and performance of partially non-glycosylated enzymes

Pyranose Dehydrogenase 1 from the basidiomycete Agaricus meleagris (AmPDH1) is an oxidoreductase capable of oxidizing a broad variety of sugars. Due to this and its ability of dioxidation of substrates and no side production of hydrogen peroxide, it is studied for use in enzymatic bio-fuel cells. In-vitro deglycosylated AmPDH1 as well as knock-out mutants of the N-glycosylation sites N75 and N175,

Going green in architectural education : An urban living lab experiment for a graduation green design studio in Saint Catherine, Egypt

Given the current energy crises, recent efforts have been directed at bringing energy efficiency and environmental awareness across the board into architectural education in Egypt. Although interest in sustainable design teaching and learning started more than 20 years ago at both post graduate and undergraduate level in Egypt, ecological illiteracy persists in architecture education. There is a l