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Climbing Fiber Coupling between Adjacent Purkinje Cell Dendrites in Vivo.

Climbing fiber discharges within the rat cerebellar cortex have been shown to display synchrony, especially for climbing fibers terminating in the same parasagittal bands. In addition, Purkinje cells which have the smallest rostrocaudal separation also seem to have the highest degree of synchrony. But this has so far only been investigated for distances down to 250 mum. In the present study, we wa

Defective secretion of islet hormones in chromogranin-B deficient mice.

Granins are major constituents of dense-core secretory granules in neuroendocrine cells, but their function is still a matter of debate. Work in cell lines has suggested that the most abundant and ubiquitously expressed granins, chromogranin A and B (CgA and CgB), are involved in granulogenesis and protein sorting. Here we report the generation and characterization of mice lacking chromogranin B (

A comparison of different purification methods of aggrecan fragments from human articular cartilage and synovial fluid.

In the study of aggrecan fragmentation several methods to extract and purify aggrecan from cartilage and synovial fluid (SF) are used. This work compares and evaluates the effectiveness for purification of aggrecan of the most commonly used methods by the ratio of sulfated glycosaminoglycan (sGAG) to protein and by fragment analysis by Western blot. A novel method for purification of aggrecan frag

Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer.

The aim of this investigation was to assess the Ku70/Ku80 complex as a potential target for antibody imaging of prostate cancer. We evaluated the in vivo and ex vivo tumor targeting and biodistribution of the (111)In-labeled human internalizing antibody, INCA-X ((111)In-DTPA-INCA-X antibody), in NMRI-nude mice bearing human PC-3, PC-3M-Lu2 or DU145 xenografts. DTPA-conjugated, non-labeled antibody

Kort telomerlängd markör för tidigt vaskulärt åldrande

Age is the most important risk factor known for cardiovascular disease manifestations. However, the biological ageing of an individual may be substantially different from the chronological age in what has been called “differential ageing”. This process can be evaluated by arterial stiffness (pulse wave velocity), and the marker of telomere length and by levels of telomerase enzymatic activity. Sev

Arsenic metabolism is influenced by polymorphisms in genes involved in one-carbon metabolism and reduction reactions.

OBJECTIVES: The susceptibility to arsenic (As)-induced diseases differs greatly between individuals, probably to a large extent due to genetic differences in arsenic metabolism. The aim for this study was to identify genetic variants affecting arsenic metabolism. METHODS: We evaluated the association between urinary metabolite pattern and polymorphisms in three gene-groups related to arsenic metab

Othering Processes in Feminist Teaching : A case study of an adult educational institution

Taking its starting point from a critical dialogue with feminist and postcolonial theory, this dissertation explores processes of othering in a feminist adult educational institution, Women’s Room, in Sweden. Women’s Room is a women-only school and half of the student body has a migrant background. The project is an ethnographic study consisting of nine months of participant observations and inter