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Antibody-based microarrays are a new powerful proteomic technology that can be used to generate rapid and detailed expression profiles of defined sets of protein analytes in complex samples as well as high-resolution portraits of entire proteomes. Miniaturized micro- and nanoarrays can be printed with numerous antibodies carrying the desired specificities. Multiplexed and ultra-sensitive assays, s

Leukotriene B-4 (LTB4) is a potent lipid mediator of inflammation that acts primarily via a seven-transmembrane-spanning, G-protein-coupled receptor denoted BLT1. Here, we describe the synthesis and characterization of fluorescent analogs of LTB4 that are easy to produce, inexpensive, and without the disadvantages of a radioligand. Fluorescent LTB4 is useful for labeling LTB4 receptors for which n

Background Ximelagatran is a novel, oral direct thrombin inhibitor under investigation as an alternative to warfarin for prevention of thromboembolism in patients with nonvalvular atrial fibrillation (AF). Two long-term studies in patients with AF and at least one additional risk factor for stroke are underway to compare the safety and efficacy of fixed-dose ximelagatran (36 mg bid) without coagu

We examined the influence of female age, male age, and pair-bond duration on start of egg-laying, clutch size, and number of young fledged in the Lesser Spotted Woodpecker (Dendrocopos minor). We also attempted to disentangle the relative influence of individual age and pair-bond duration on reproduction, because the effect of those factors may be confounded. Brooding performance improved with age

Detta är ett kapitel i en volym vars syfte är att studera det fantastiska i litteratur från olika delar av den fransktalande världen. Mitt bidrag handlar om fallet Patrick Chamoiseau som ett exempel på modern användning av det fantastiska i västindisk litteratur.This is a chapter in a volume whose aim is to study the fantastic in literature from different parts of the French speaking world. My contribution deals with the case of Patrick Chamoiseau as an example of the modern use of the fantastic in Carribean literature.

OBJECTIVE: To compare 3 immunoassays, an immune complex assay, and an application of the polymerase chain reaction (PCR) for the diagnosis of tuberculous meningitis (TBM). MATERIAL: Cerebrospinal fluid (CSF) from 33 patients with TBM and from 34 control patients with infectious and non-infectious CNS diseases was analysed. RESULTS: The antibody immunoassays were either nonspecific or insensitive.

Antibody-based microarrays are a novel technology that hold great promise in proteomics. Microarrays can be printed with thousands of recombinant antibodies carrying the desired specificities, the biologic sample (e.g., an entire proteome) and any specifically bound analytes detected. The microarray patterns that are generated can then be converted into proteomic maps, or molecular fingerprints, r

Pili of Neisseria gonorrhoeae are phase-variable surface structures that mediate adherence to host target cells. Each pilus is composed of thousands of major pilus subunits, pilins, pilus-associated protein PilC, and possibly other components. Piliated and nonpiliated gonococcal clones may secrete a soluble smaller pilin (S-pilin) that is cleaved after amino acid 39 of the mature pilin protein. He

Helicobacter pylori is able to utilize several lectin-like, protein-carbohydrate interactions for binding to mucins, cell surfaces, and extracellular matrix proteins. As determined by hemagglutination assays and binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms, strains of gastric helicobacters and enterohepatic helicobacters, including Helicobacter canis, Helicoba

Denkpsychologie has been important for the development of psychology as well as of philosophy during the last century. More specifically, cognitive psychology as well as Karl Popper's evolutionary epistemology were both influenced by Otto Selz's cognitive psychology. Without doubt, Selz played a role in the development of Popper's thinking, but Michel ter Hark has not given convincing evidence for

A combination of hydrogen/deuterium (H/D) exchange and limited proteolysis experiments coupled to mass spectrometry analysis was used to depict the conformation in solution of HAMLET, the folding variant of human alpha-lactalbumin, complexed to oleic acid, that induces apoptosis in tumor and immature cells. Although near- and far-UV CD and fluorescence spectroscopy were not able to discriminate be

This article sets out to describe and explain the events that led, in the summer of 1967, to political chaos and near civil war in many parts of China. It links the violence on the ground to statements and policies formulated at the highest levels of the CCP and sets out to show how and why Mao Zedong himself must bear direct personal responsibility for what stands out as one of the darkest chapte

The molecular conservation of a surface-exposed lipoprotein, protein D, of Haemophilus influenzae was studied by cloning and sequencing of the gene encoding protein D from three encapsulated type b strains and three nontypeable strains of H. influenzae. These nucleotide sequences were analyzed with previously reported sequences from one type b strain and one nontypeable strain. The nucleotide sequ