Optimizing the Purification of the Painless Protein
The mosquito Aedes aegypti is a known vector for many diseases, which leads to the use of harmful insecticides. Painless is a membrane protein that plays a role in sensing noxious heat mechanical stimuli, and by determining its structure, it could potentially be a target repellents as an alternative to insecticides. By expressing Painless an additional eGFP HisTag in yeast cells, the protein can