Alternative ways to cultivate and challenge a myeloid cell model applied in immunotoxicity testing
There is a strong need to replace animal-based experiments with in vitro alternatives. Mutz-3, a myeloid cell line, has been successfully used to investigate skin sensitization. A problem with in vitro assays, not only affecting Mutz-3, is the use of Fetal Bovine Serum for cell cultivation, as it is a serum supplement which is not fully defined, and it introduces variability in cell-based experime
