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The influence of different pressures of CO2 and the addition of caprylic acid methyl ester as an entrainer was studied for the extraction process of borage seed. The increase of CO2 pressure from 100 to 350 bar resulted in the increase in extract yield from 0.14 to 24.29% (w/w) while the changes in the extract composition were not so considerable. The highest solubility of pure caprylic acid methy

Background 3-Hydroxy fatty acids (3-OHFAs), components of lipid A of gram-negative bacteria are useful chemical markers of endotoxin. Methods We analyzed 3-OHFAs in house dust as trimethylsilyl (TMS) methyl ester derivatives in the electron impact ionization mode using gas chromatography ion-trap mass spectrometry. Linear calibrations with r > 0.995 were observed for all the 3-OHFA methyl ester ex

Rationale, aims and objective The objectives were to describe and evaluate the structured medication questionnaire and to improve data handling of results from the Morisky four-item scale for patient compliance and Beliefs about Medicines Questionnaire-specific (BMQ-specific). Methods A questionnaire was developed with the purpose of being used when identifying medication errors and assessing pati

In the process of making ethanol from lignocellulosic materials, compounds inhibitory to microorganisms are generated during steam pretreatment of the wood. Water-soluble inhibitors and pentoses are liberated and washed from the cellulose structure which is further enzymatically hydrolyzed. To make the process economically feasible, rite pentoses have to be fermented to ethanol. A major drawback w

Soil chemistry models often use gibbsite solubility and similar equilibrium models to predict Al concentrations in soil solution. A kinetic alternative was developed with the goal of finding universal rate constants instead of the site- and depth-specific solubility constants usually associated with the equilibrium approach. The behavior of the two approaches was studied within the framework of th

Immunohistochemistry has been used to demonstrate that neuropeptide Y, dopamine-beta-hydroxylase, calcitonin gene-related peptide or substance P are colocalized with vasoactive intestinal polypeptide and choline acetyltransferase in subpopulations of neurons in cranial parasympathetic ganglia of rat. These comprise the ciliary, sphenopalatine, otic, glossopharyngeal-vagal and internal carotid gang

A method for simultaneous extraction and quantification Of four non-steroidal anti-inflammatory drugs (NSAIDs) based on continuous flow hollow fiber liquid-phase microextraction (CFHF-LPME) was developed. The effect of sample flow rate, acceptor flow rate, type of acceptor flow (continuous, semi-continuous or forward-backward), type of supported liquid membrane and sample volume was studied. The e

Breast cancer is the most common disease in women, and up to 38% develop lymphedema of the arm following mastectomy, standard axillary node dissection and postoperative irradiation. Limb reductions have been reported utilizing various conservative therapies such as manual lymph and pressure therapy. Some patients with long-standing pronounced lymphedema do not respond to these conservative treatme

he North Taymyr ice-marginal zone (NTZ) is a complex of glacial, glaciofluvial and glaciolacustrine deposits, laid down on the northwestern Taymyr Peninsula in northernmost Siberia, along the front of ice sheets primarily originating on the Kara Sea shelf. It was originally recognised from satellite radar images by Russian scientists; however, before the present study, it had not been investigated

Background and aims: As part of an RCT in six European sites, the direct mental health care cost for 422 patients with schizophrenia was analysed according to how total and medication costs differed across sites and which variables were likely to predict total or service-specific costs. Method: Service use was recorded continuously during a 12-month follow-up. Prescribed psychotropic medication wa

Efficient screening of ligands interacting with G-protein-coupled receptors is central for modern drug development. Here, we describe an optimized reporter vector primarily intended for use in reporter cell lines expressing such receptors. The construct consists of a synthetic enhancer containing 9x TRE (12-O-tetradecanoylphorbol-13-acetate-responsive elements) fused to a minimal CMV (cytomegalovi

We have identified for the first time an age-dependent spontaneous loss of tolerance to two self-antigenic epitopes derived from putative diabetes-associated antigens glutamic acid decarboxylase (GAD65) and glial fibrillary acidic protein (GFAP) in RIP-B7/DRB1*0404 HLA transgenic mice. Diabetic and older non-diabetic mice exhibited a proliferative response to an immunodominant epitope from GAD65 (

Cocaine- and amphetamine-regulated transcript (CART) peptide, originally isolated from brain, is also expressed in the peripheral nervous system. The distribution, origin and projections of CART-expressing enteric neurones by immunocytochemistry and in situ hybridization in rat gastrointestinal (GI) tract were studied. Possible motor functions of CART were studied in vitro using longitudinal muscl

The transport of macromolecules during peritoneal dialysis is highly selective when they move from blood to dialysate but nearly completely unselective in the opposite direction. Aiming at describing this asymmetry, we modeled the peritoneal barrier as a series arrangement of two heteroporous membranes. First a three-pore membrane was considered, crossed by small [radius of the small pore (r(s)) a

Aims: To determine the effects of a nurse-led intervention designed to improve self-management of patients with heart failure in a primary health care setting regarding health-related quality of life and depression. Methods: Patients at eight primary health care centres were screened by the Diagnosis Related Groups registry for the diagnosis of heart failure and eligibility for a cluster randomise

Detailed mineral magnetic measurements were carried out on three varved lake-sediment sequences in Sweden, which extend to ca. 7000 BC. The comparison of the magnetic properties of the organic rich varved lake-sediments with their respective catchment materials indicates that the magnetic properties of the sediments are dominated by relatively high concentrations of single-domain magnetite magneto