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A method for high throughput screening of Green Fluorescent Proteins carrying metal binding tags in bacteria was developed. A random four amino acids tag-peptide library was successfully generated in E. coli. A 96-microtiter plate assembled with metal-iminodiacetic acid small cryogel columns was used for library screening. For the first time we were able to simultaneously screen a metal binding pe

Progression through the plant life cycle involves change in many essential features, most notably in the capacity to reproduce. The transition from a juvenile vegetative and non-reproductive to an adult reproductive phase is gradual and can take many years; in the conifer Norway spruce, Picea abies, typically 2025 years. We present a detailed analysis of the activities of three regulatory genes wi

Palladium supported on Si-stabilised alumina has been demonstrated to be an active and durable catalyst for the combustion of methane. Si is more effective in stabilising alumina than La or Ba when the elements are added through an impregnation technique. Multiple stabilisation with combinations of La, Ba and Si does not increase further the stability against sintering. The stability increases log

The paper presents expressions for the point mobility of infinite plates driven by a completely rigid indenter. The problem is of general interest in connection with the excitation and transmission of structure-borne sound. The indenter is assumed to be circular, weightless, and stiff compared with the plate. A rigid indenter is assumed to provide a better approximation of the actual situation tha

Purpose: Fibroblasts are functionally diverse and fibroblastic cells with smooth muscle-like characteristics (myofibroblasts) regulate smooth muscle activity in certain tissues. The presence of myofibroblasts has been reported in the bladder with important implications for normal function and detrusor overactivity. We assessed fibroblastic cell characteristics to discern features suggesting a myof

Design is the concept of fashion. This article is focusing on the notion that there is one ideal design of society. The search for ideal solutions of societal problems has been the habit of utopianism. With the purpose to clarify how a student understands prescription and the design of a perfect society, a discourse on Huxley’s ”Brave New World” is studied. The student as text producer is expected

In recent years, the advent of experimental methods top robe gene expression profiles of cancer on a genome-wide scale has led to widespread use of supervised machine learning algorithms to characterize these profiles. The main applications of these analysis methods range from assigning functional classes of previously uncharacterized genes to classification and prediction of different cancer tiss

Herpes simplex virus (HSV) infected cells express on their surface a receptor with affinity to the Fc portion of immunoglobulin G (IgG). The influence of the infected host cell on the specificity of the receptor was investigated with radiolabeled human IgG Fc fragment and four animal IgGs (rabbit, dog, cow, and rat) and eight human and animal (primate, rabbit, dog, cow, and rat) fibroblastoid and

In this paper streamwise and transverse oscillations of a rigid circular cylinder in a rectangular channel are investigated using the Virtual Boundary method. Numerical study of the three-dimensional flow around the cylinder is done for the uniform Cartesian grid. All computations are performed at Reynolds numbers of Re=500 and 1000 based on the mean flow velocity. The Keulegan-Carpenter number (K

An in-depth photoionization study of the inner-valence electrons in HCl and DCl has been performed using synchrotron radiation. A series of photoelectron spectra of HCl were obtained at a resolution of 23 meV over the binding energy range 25-30.5 eV at various excitation energies and at two different electron collection angles relative to the plane of polarization of the undulator radiation. In ad

S-(4-hydroxybenzyl)glutathione was isolated as the major principle responsible for the inhibition of the in vitro binding of kainic acid to brain glutamate receptors by water extracts of the plant Gastrodia elata. The affinity (IC50 value) of the compound is slightly lower compared to glutamate and glutathione.

The role of attention in speeded Garner classification of concurrently presented auditory and visual signals was examined in 4 experiments. Within-trial interference (i.e., congruence effects) occurred regardless of the attentional demands of the task. Between-trials interference (i.e., Garner interference) occurred only under conditions of divided attention when making judgments about auditory si

Hypersecretion of airway mucus is a characteristic feature of chronic airway diseases like cystic fibrosis (CF) and leads via impairment of the muco-ciliary clearance and bacterial superinfection to respiratory failure. The major components of the mucus matrix forming family of mucins in the airways are MUC5AC and MUC5B. To investigate the expression of these glycoproteins in CF, immunohistochemis

Popular Abstract in Swedish Automatisk analys av programkod är ett kärnområde inom datavetenskap. Det mest typiska verktyget inom detta område är en kompilator som översätter källkod till maskinkod, men det finns många likartade verktyg, exempelvis översättning mellan språkdialekter, kontroll av kodkonventioner samt automatisk omstrukturering av kod. Eftersom dessa verktyg gör liknande analyser fiProcessing of programs is a core area in computer science. A compiler that translates source text to machine language is the most well-known kind of tool in this area, but there are numerous other kinds of related applications: source-to-source translators, refactoring tools, reengineering tools, metrics tools, consistency checkers, etc. These tools perform similar analyses and can therefore benef

Popular Abstract in Swedish Under det senaste decenniet har föräldrars intresse att välja fristående skolor för sina barn ökat markant. Läsåret 1998-99 gick, enligt Skolverket (1999), drygt 30 000 eller 3 % av grundskolans elever i fristående skolor. Det är emellertid en stor variation mellan landets kommuner. Trots den ringa andel som eleverna i fristående skolor utgör, har diskussionen om skolorAbstract Although the number of children attending private schools in Sweden is less than 4% of those attending compulsory schools, the debate of these schools has been intense. Apart from details as fees and funding, the role of education’ in the Swedish welfare system has been subject of much debate. In spite of the debate, in Sweden there is a lack of knowledge about private schools. The aim of