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Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer wa
Molecularly imprinted polymers are highly stable and can be sterilised, making them ideal for use in biotransformation process. In this communication, we describe a novel application of molecularly imprinted polymers in an enzymatic reaction. The enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to give Z-L-Asp-L-Phe-OMe (Z- aspartame) was chosen as a model system to ev
In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micronscale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding ana
We have studied the possibility of shifting a thermodynamically unfavourable enzymatic equilibrium towards product formation via the addition of a highly specific adsorbent. The commercially interesting enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to the sweetener aspartame was chosen as the model system. Extremely stable and specific adsorbents for the product Z-L
Molecular imprinting is an attractive method for producing highly selective adsorbents, and several new and potentially useful applications based on molecularly imprinted polymers (MIPs) have been described in recent years. In this article, we highlight some of the areas where these materials have found application, and also describe some new fields of application where the selectivities of imprin
Molecularly imprinted polymers were prepared using 2-vinylpyridine and/or methacrylic acid as functional monomers in a self-assembly imprinting protocol. The resulting polymers were analyzed in aqueous media, and the effects from the pH of the mobile phase and the degree of added organic solvent were investigated. The results are indicative of the importance of ionic bonds in conjunction with hydr
In this study, both diastereo- and enantioselective adsorbents for a dipeptide derivative were prepared using a molecular imprinting technique. The diastereo- and enantioisomers for the dipeptide derivative N-(benzyloxy-carbonyl)aspartylphenylalanine methyl ester (ZAPM), in addition to the α- and β-isomers, were chosen as test compounds for the investigation of the imprinting effect The close simi
Molecular imprinting is an attractive method for producing highly selective adsorbents, and several new and potentially useful applications based on molecularly imprinted polymers (MIPs) have been described in recent years. In this article, we introduce a new field of application in which the selectivities of imprinted materials can be gainfully employed as binding matrices in the screening of com
Molecular imprinting has gained increasing research interest during the past few years. In this overview we would like to explain to the readers how a seemingly simple concept can eventually lead to useful applications in several areas. The main focus will be on the present state of the art of molecular imprinting, as several breakthroughs have occurred since most reviews by us and other groups ha
A novel method for the preparation of molecularly imprinted microspheres has been developed. Theophylline, caffeine, and 17β-estradiol are used as template molecules to demonstrate the generally applicable methodology. In the presence of a template molecule, cross-linking polymerization starting from a dilute solution of methacrylic acid and trimethylolpropane trimethacrylate yields polymer micros
Protein-coding genetic variants that strongly affect disease risk can yield relevant clues to disease pathogenesis. Here we report exome-sequencing analyses of 20,791 individuals with type 2 diabetes (T2D) and 24,440 non-diabetic control participants from 5 ancestries. We identify gene-level associations of rare variants (with minor allele frequencies of less than 0.5%) in 4 genes at exome-wide si
Objectives: Moderate physical activity gains survival. There are, however, several variables that may affect this relationship. In this study, the relationship between moderate physical activity and longevity was investigated, taking into account age, gender, smoking habits, cohabitation status, body mass index, leg strength and balance, education level and cognitive function. Method: A sample of
The history of commercial exploitation of fish stocks is replete with instances of over-exploitation and stock collapse. Particularly in situations where little is known about a species or a particular fish stock, unregulated expansion into new fisheries may effectively wipe out a species or stock before its existence is even formally recognised or understood. Globally, there has been a strong int
