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Five month persistence of Helicobacter pylori infection in guinea pigs

Seven Dunkin-Hartley guinea pigs were infected with the Sydney strain of H. pylori (SS1). Gastric histopathology was evaluated and serum antibody response to H. pylori cell-surface proteins was analysed by enzyme immunoassay (EIA) and immunoblot. Tissue and faecal samples from five control animals were analysed for the presence of naturally occurring Helicobacter spp. infection by culture and Heli

Association between urinary N-acetyl-beta-glucosaminidase and its isoenzyme patterns and microangiopathy in type 1 diabetes mellitus

Urinary N-acetyl-beta-glucosaminidase (NAG) and its isoenzymes (NAG A and NAG B) in samples from 87 type 1 diabetic patients and 40 apparently healthy reference subjects were studied with enzyme immunoassays. The diabetic patients had higher concentrations of urinary NAG than did the control subjects (P less than 0.01), but the isoenzyme pattern did not differ. There was a positive correlation bet

Ultrafiltration of effluents front chlorine-free, kraft pulp bleach plants

Ultrafiltration has proven to be a useful technology for treating alkaline bleach plant filtrates in sulphite pulp mills. In this investigation the treatment of seven alkaline filtrates from kraft pulp mills were studied. The results include high fluxes and fair, or even good, removal of organic, as well as inorganic, substances. The flux seems to depend on the concentration of the bleach plant fi

Adhesion of the probiotic bacterium Lactobacillus plantarum 299v onto the gut mucosa in critically ill patients: a randomised open trial.

Introduction To achieve any possible positive effect on the intestinal mucosa cells it is important that probiotics adhere tightly onto the intestinal mucosa. It has been shown in healthy volunteers that Lactobacillus plantarum 299v (Lp 299v) (DSM 9843), a probiotic bacterium, given orally in a fermented oatmeal formula adheres onto the intestinal mucosa, but whether this also occurs in critically

Diurnal and vertical variability of the sensible heat and carbon dioxide budgets in the atmospheric surface layer

The diurnal and vertical variability of heat and carbon dioxide (CO2) in the atmospheric surface layer are studied by analyzing measurements from a 213 m tower in Cabauw (Netherlands). Observations of thermodynamic variables and CO2 mixing ratio as well as vertical profiles of the turbulent fluxes are used to retrieve the contribution of the budget terms in the scalar conservation equation. On the

Transcriptome and proteome analysis of soleus muscle of hormone-sensitive lipase-null mice

Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization in adipocytes, has been demonstrated also in skeletal muscle. To gain further insight into the role and importance of HSL in skeletal muscle, a transcriptome analysis of soleus muscle of HSL-null mice was performed. A total of 161 transcripts were found to be differentially expressed. Increased mRNA levels of fructose-1,6-bisp

Experimental investigation of local heat transfer in a square duct with various-shaped ribs

In the present study, experimental studies are carried out to investigate the heat transfer and friction characteristics in a square duct roughened by various-shaped ribs on one wall. The ribs are oriented transversely to the main stream in a periodic arrangement. Liquid crystal thermography is employed to measure the local and average heat transfer coefficient on the ribbed surface. The rib heigh

Surfactant-Promoted Reductive Synthesis of Shape-Controlled Gold Nanostructures

We describe a surfactant-promoted reductive route for the shape-controlled synthesis of gold nanostructures by hydrothermal treatment of chloroauric acid in the presence of the surfactant hexadecyltrimethylammonium bromide (CTAB) without using reducing agent. The results show that the cationic surfactant CTAB provides the dual function of promoting Au-III reduction to Au-0 and size- and shape-cont

Plant species diversity, plant biomass and responses of the soil community on abandoned land across Europe: idiosyncracy or above-belowground time lags

We examined the relationship between plant species diversity, productivity and the development of the soil community during early secondary succession on former arable land across Europe. We tested the hypothesis that increasing the initial plant species diversity enhances the biomass production and consequently stimulates soil microbial biomass and abundance of soil invertebrates. We performed fi

Effects of CO2 and light on tree phytochemistry and insect performance

Direct and interactive effects of CO2 and light on tree phytochemistry and insect fitness parameters were examined through experimental manipulations of plant growth conditions and performance of insect bioassays. Three species of deciduous trees (quaking aspen, Populus tremuloides: paper birch, Betula papyrifera; sugar maple, Acer saccharum) were grown under ambient (387 +/- 8 mu L/L) and elevate

The impact of translocations and gene fusions on cancer causation.

Chromosome aberrations, in particular translocations and their corresponding gene fusions, have an important role in the initial steps of tumorigenesis; at present, 358 gene fusions involving 337 different genes have been identified. An increasing number of gene fusions are being recognized as important diagnostic and prognostic parameters in malignant haematological disorders and childhood sarcom

Microbial characteristic of soils on a latitudinal transect in Siberia

Soil microbial properties were studied from localities on a transect along the Yenisei River, Central Siberia. The 1000 km-long transect, from 56°N to 68°N, passed through tundra, taiga and pine forest characteristic of Northern Russia. Soil microbial properties were characterized by dehydrogenase activity, microbial biomass, composition of microbial community (PLFAs), respiration rates, denitrifi

The calpain inhibitor VI prevents apoptosis of adult motor neurons

Motor neuron cell death was studied in organotypic cultures of adult spinal cord slices from the mouse. Six hours after excision, many motor neuron nuclei displayed apoptotic features including nuclear and chromatin condensation. At this time point, many motor neurons also exhibited immunoreactivity to calpain II. Both the calpain inhibitor VI and ethyleneglycol-bis(beta-aminoethyl ether) N,N,N',N

Calibration services in the frame of accredited coagulation EQA schemes - Meeting the IVD directive

Organisers of external quality assurance programmes may, for various reasons, also manufacture in vitro diagnostic medical devices, or provide calibration services. This paper describes measures taken by a Swedish organiser to meet the requirements of the European Commission's directive 98/79/EC for a national calibration of prothrombin time. Quality management system requirements and interactions

Expression and signaling activity of Wnt-5a/discoidin domain receptor-1 and Syk plays distinct but decisive roles in breast cancer patient survival

Purpose: The loss of Wnt-5a, a G-protein-coupled receptor ligand, or Syk, an intracellular kinase, has in separate studies been associated with poor prognosis of breast cancer patients. Both proteins are involved in cell adhesion, a key event in epithelial cancer metastasis. Here, we have investigated whether Syk is part of the Wnt-5a/discoidin domain receptor-1 (DDR1) signaling pathway and if a s

Collagen type II is recognized by a pathogenic antibody through germline encoded structures

Collagen type II (CII) is a cartilage-specific target of pathologic humoral autoimmune responses in rheumatoid arthritis as well as in the collagen-induced arthritis model. The aim of the present study is to investigate the critical amino acid residues conferring CII epitope specificity of the prototypic arthritogenic murine mAb CIIC1. A homology model of the CIIC1 single-chain antibody fragment (

Towards a neuroprotective gene therapy for Parkinson's disease: use of adenovirus, AAV and lentivirus vectors for gene transfer of GDNF to the nigrostriatal system in the rat Parkinson model

During the last few years, recombinant viral vectors derived from adenovirus (Ad), adeno-associated virus (AAV) or lentivirus (LV) have been developed into highly effective vehicles for gene transfer to the adult central nervous system. In recent experiments, in the rat model of Parkinson's disease, all three vector systems have been shown to be effective for long-term delivery of glial cell line-

Review of uppermost Furongian trilobites from Scania, Southern Sweden, based on type material

The Scandinavian trilobites described in the classical papers by Moberg and Moller (1898) and Moberg (1898) are reviewed, based on the original type collections. The ten species and subspecies originally described are here considered to represent 12 species and subspecies, ranging in age from the latest Furongian (late Cambrian) through the Tremadocian (Early Ordovician). Lectotypes are selected f