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Transient kinetics of the equine infectious anemia virus deoxyuridine 5'-triphosphate nucleotide hydrolase were characterized by monitoring the fluorescence of the protein. Rate constants for the association and dissociation of substrate and inhibitors were determined and found to be consistent with a one-step mechanism for substrate binding. A C-terminal part of the enzyme presumed to be flexible

The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at di

Model systems of cross-linked polyelectrolyte gels were investigated by means of Monte Carlo simulations. The model contained a charged defect-free th ee-dimensional network of a diamond-like topology and explicit counterions. Pressure vs volume relations and chain extensions were determined and compared to those of the corresponding polyelectrolyte solution. The structure of the gel was character

In mobile ad hoc networks, nodes are often modeled as having the same capabilities. However, there are many cases in which certain nodes host special services to other nodes in the network. Examples of such services are Internet access, DNS proxy or AAA servers. We refer to these special nodes as netmarks. Because there is a high likelihood that netmarks will communicate with the rest of the netwo

Analys av visuella berättarmönster i Hitchcocks filmer med fokus på en jämförelse mellan Hitchcocks Psycho (1960) och Gus Van Sants nyinspelning (1998).

In this study a set of structures has been simulated to represent a range of clinically relevant breast cancer mammographic lesions including solid tumours and microcalcifications. All structures have been created using simple random-based mathematical functions and have been inserted into a subset of digital mammography images at appropriate contrast levels into various regions of the breast, inc

The binding of SDS to cellulose polymers in the semidilute concentration regime has been studied by means of NMR, ion-selective electrode, and a time-resolved fluorescence technique. Two polymers have been used, differing only in a low degree of hydrophobic modification of one of them. NMR self-diffusion and activity measurements show that the binding of SDS to the nonmodified polymer has a fairly

OBJECTIVE: Nitric oxide (NO) and oxygen free radicals are implicated in the pathophysiology of traumatic brain injury (TBI). Peroxynitrite formation from NO and superoxide contributes to secondary neuronal injury but the neuroprotective effects of nitric oxide synthase (NOS)-inhibitors have been contradictory. This study was undertaken to examine whether PTtic administration of the (NOS)-inhibitor

This article outlines an experimental and numerical study of self-desiccation and chemical shrinkage in Portland cement mortars, with or without silica fume. For this purpose 9 different cement mortars were cast in 90 pycnometers (study of chemical shrinkage and hydration) or 180 glass flasks (study of self-desiccation and hydration). Chemical shrinkage was observed for 14 days and self-desiccatio

BACKGROUND: In experimentally induced myocardial infarction, mild hypothermia (33-35 degrees C) is beneficial if applied prior to ischemia or reperfusion. Hypothermia, when applied after reperfusion seems to confer little or no benefit. The mechanism by which hypothermia exerts its cell-protective effect during cardiac ischemia remains unclear. It has been hypothesized that hypothermia reduces the

After obtaining informed consent, we randomized 78 patients with severe hemiparesis of the left or right side within 10 days of stroke onset: 40 to a control group receiving daily physiotherapy and occupational therapy, and 38 to a group that, in addition, we treated with sensory stimulation (acupuncture) twice a week for 10 weeks. The median age was 76 years for both groups. Motor function, balan

The heat produced by the metabolism of a mould fungus (Penicillium brevicompactum) growing on softwood was measured by isothermal calorimetry under different relative humidity (RH) levels during desorption (from 95% to 65% RH) and absorption (from 65% to 95% RH). The humidification method was successful and it was possible to decrease and increase the relative humidity and moisture content in smal