At CTG, we offer both sequencing-based and imaging-based spatial applications. These technologies provide comprehensive and high-resolution insights into tissue architecture, gene and protein expression. We offer 10x Genomics Visium v2, Visium HD on the CytAssist, and Xenium (coming Q3 2025, book consultations now!). Our workflows are compatible with FFPE tissue sections and can integrate high-res

https://www.ctg.lu.se/services/spatial - 2025-06-17

Visium v2 and Visium HD for FFPE tissue are probe-based spatial transcriptomics solutions for high-resolution gene expression analysis. Both kits can be combined with protein staining enables whole-transcriptome profiling with spatially barcoded probes and limited protein co-detection, including a panel for 31 immune proteins. Visium HD offers enhanced spatial resolution for deeper insights into t

https://www.ctg.lu.se/services/spatial/visium-v2-visium-hd - 2025-06-17

Xenium (Launching Q3 2025) enables high resolution through in situ sequencing with a predefined gene panel, offering cell segmentation. This cutting-edge approach allows for subcellular resolution, providing unparalleled insights into the spatial distribution of transcripts within specific cellular compartments. With Xenium, researchers can achieve precise cell segmentation and detailed analysis o

https://www.ctg.lu.se/services/spatial/xenium-situ-sequencing - 2025-06-17

The Center for Translational Genomics (CTG) was established in 2017 as a prioritized research infrastructure and open core facility within the Faculty of Medicine at Lund University specialized in providing expertise and services in advanced Next Generation Sequencing-based technologies, aiming to enable groundbreaking and innovative research in molecular life sciences. CTG collaborates closely wi

https://www.ctg.lu.se/welcome-ctg - 2025-06-17

18 March 2025 14:00 to 15:00 | Other NBIS (National Bioinformatics Infrastructure Sweden) provides bioinformatics and data science support to the Swedish life science research community. Weekly drop-ins are open for anyone doing academic research with questions about bioinformatics! Are you planning a new project? Are you stuck in some part of your analysis? Do you need help interpreting the resul

https://www.ctg.lu.se/calendar/nbis-drop-online - 2025-06-17

19 March 2025 10:30 to 11:30 | Other NBIS (National Bioinformatics Infrastructure Sweden) provides bioinformatics and data science support to the Swedish life science research community. Weekly drop-ins are open for anyone doing academic research with questions about bioinformatics! Are you planning a new project? Are you stuck in some part of your analysis? Do you need help interpreting the resul

https://www.ctg.lu.se/calendar/nbis-drop-lund - 2025-06-17

7 April 2025 12:00 to 16:30 | Seminar Take your RNA Gene Expression Research to the Next Level! Join our seminar to learn how to optimize your RNA analysis.This session is designed for researchers, graduate students, and laboratoryprofessionals looking to enhance their expertise.Topics Covered:RNA extraction and Quality ControlNGS Library PrepBiox Data AnalysisLunch will be served at 12:00. Regist

https://www.ctg.lu.se/calendar/sample-insight-qiagen - 2025-06-17

High-throughput sequencing at cost-effective pricing Available KitsEach kit is defined by the number of reads (single-end or clusters) and read length (determined by cycle count).e.g. 10B, 300 cycle flowcell will deliver about 10 billion single-end reads sequenced with the following read structure: 150-10-10-150Sample Volume Requirements1.5B Flow Cell (2 lanes): 100 µl per lane (min 50 µl)10B Flow

https://www.ctg.lu.se/services/sequencing/novaseq-x-plus - 2025-06-17

Mid-throughput sequencing for maximum flexibility Available Kits - XLEAP-SBS Chemistry only.Each kit is defined by the number of reads (single-end or clusters) and read length (determined by cycle count).e.g. P4, 300 cycle flowcell will deliver about 1.8 billion single-end reads sequenced with the following read structure: 150-10-10-150Sample Volume RequirementsAll flow cells (P1, P2, P3, P4): 60

https://www.ctg.lu.se/services/sequencing/nextseq-2000 - 2025-06-17

Welcome to CTG! Center for Translational Genomics (CTG) is a research infrastructure and technical platform for Next Generation Sequencing (NGS) and other genomics technologies at the Medical Faculty of Lund University. Certified Service Providers for:    Xenium Services Launching Soon! We’re excited to announce that our Xenium services will be launching in Q3! Get ahead by contacting us for consu

https://www.ctg.lu.se/ctg-center-translational-genomics - 2025-06-17

We offer various DNA sequencing services, including Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), Targeted Gene Sequencing, Methylome Sequencing, Cell-Free DNA (cfDNA), and Small Genome Sequencing. All libraries are sequenced on Illumina platforms (NovaSeqXPlus or NextSeq2000). Unless specified otherwise, we use adapters with UMI and Unique Dual Indexes from IDT. Turnaround Time:Dat

https://www.ctg.lu.se/services/genomics - 2025-06-17

Molarity and amounts of library pool for sequencing Dilute library pool in EB buffer 10mM Tris-Cl, pH 8,5. Lower concentrations than stated below can be discussed. For NovaSeq: Molarity and volume depend on type of library and flowcell Unique dual index Delivery to CTG Fill in OrderForm, pool information, and SampleSheet for Illumina (csv file) and send to your contact at CTG.   Contact Informatio

https://www.ctg.lu.se/genomic-services/sequencing-only/sample-requirement - 2025-06-17

Our bioinformatics services include below analysis pipeline, deliverables and data management. Other pipelines can be discussed. Seq-Only Analysis pipelineDemultiplexing, involves in re-organizing the FASTQ files based on the sample index information, and generating the statistics and reporting files. This task will be performed using the bcl2fastq2 software with default settings.DeliverablesProje

https://www.ctg.lu.se/genomic-services/sequencing-only/bioinformatics-and-deliverables - 2025-06-17

DNA ExtractionWe recommend Qiagen spin column-based nucleic acids purification methods. Concentration and PurityUse spectrophotometrical method to get the concentration and a ratio (OD260/280 and OD260/230) of each sample.DNA should have an OD260/280 ratio ~1.8 and OD260/230 ratio in range 1.8-2.2 (the ratio shows purity of samples).DNA amount recommendedMethylation: Bisulfitconversion: 555 ng DNA

https://www.ctg.lu.se/genomic-services/array/sample-requirement - 2025-06-17

Our bioinformatics services include below analysis, deliverables and data management. AnalysisQC and bioinformatics analysis is done in Illuminas Genome Studio software.DeliverablesExcel file summarizing information about the experimental setup and methods (MIAME.xls)5 folder:DecodeData – files used during scanning.GenomeStudio – executable file (.bsc) for the created GenomStudio project.ImageData

https://www.ctg.lu.se/genomic-services/array/bioinformatics-and-deliverables - 2025-06-17

This service is only for future sequencing projects at CTG. Requesting a project at CTGTo initiate a project using our available services, please start a project in iLab. You can login using your LucatID (SWAMID) on the Lund University iLab webpage.  RNA RNA concentration RNA Concentration is measured by a fluorometric method, using the Qubit 4 or Qubit Flex instruments. Available Assays: Broad Ra

https://www.ctg.lu.se/services/quality-control - 2025-06-17

Service projects are registered and processed in iLab, an online project managing software for core facilities. It provides transparency of projects and quotes. It also includes a booking schedule for some equipment. What is iLab?iLab is our core facility's online platform for managing sequencing service orders. Through iLab, you can:Request project consultationsGet price quotesInitiate library pr

https://www.ctg.lu.se/how-start-project - 2025-06-17

CTG has a wide range of services which include laboratory analysis and bioinformatics pipelines. We also offer advice on how to design your research project to allow for reliable results. Genomics Single Cell Transcriptomics Spatial Sequencing Quality Control

https://www.ctg.lu.se/services - 2025-06-17

Special Offers for New Users! Trying Parse Evercode for the first time? Enjoy up to 45% off your first run:Mini: 45% off, WT: 18% off, Mega 96: 8% off, Mega 384: 5% off Since 2019, CTG has offered a broad range of single-cell applications, providing expert knowledge, accessibility, and personalized support. We continuously update our methods and instruments in collaboration with local researchers,

https://www.ctg.lu.se/services/single-cell - 2025-06-17

The Universal kits (formerly Next GEM Gene Expression) offer scalable whole transcriptome profiling at the single-cell level, with flexible sample input options. The 3' assay can be paired with cell surface protein staining, while the 5' assay supports full-length TCR/BCR sequencing, antigen specificity, CRISPR guide analysis, and cell surface protein profiling. For cost efficiency, samples can be

https://www.ctg.lu.se/services/single-cell/universal-3-or-5-gene-expression - 2025-06-17