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Educational Collaboration Aiming at Effective Communication

Resorting more and more to e-learning means a thorough transformation of the teaching processes. Once the teacher-student communication is realized on yahoo-groups or Facebook-groups, the classical roles in the classroom become obsolete. With teachers having the possibility to enter in due time on students’ forums, the former relation of power in education moves to a collaborative one. More and mo

Simple and accurate four-node axisymmetric element

Based on the assumed strain method, a simple four-node axisymmetric solid element is introduced. The assumed strain field is carefully selected to preserve the correct rank of the element stiffness matrix and to achieve high accuracy. The strain field is developed in conjunction with orthogonal projections and no matrix inversions are needed. The coarse mesh accuracy in bending and in typical axis

Agro-biomass-to-energy systems in transition economies: cases of Ukraine and Lithuania

Economies in transition (EiTs) (e. g. Ukraine (UA), Belarus, Lithuania (LU), Moldova, etc.) face broad needs for energy security enhancement, energy diversification, revitalisation of agriculture and improvements in the state of the environment. Development of national bioenergy potentials can contribute to delivering solutions to these problems. However, EiTs face technical, political, financial,

Label-free acoustophoretic enrichment of mononuclear cells from blood

We are reporting an efficient, label-free and continuous separation of mononuclear cells (MNC), from blood using microchip acoustophoresis. In standard PBS buffer, MNCs and red blood cells (RBC) display overlapping acoustophoretic mobilities which compromise separation of these cell types from each other. In this paper we capitalize on the fact that MNC and RBC display different acoustophysical pr

Human radical scavenger α1-microglobulin protects against hemolysis in vitro and α1-microglobulin knockout mice exhibit a macrocytic anemia phenotype

During red blood cell (RBC) lysis hemoglobin and heme leak out of the cells and cause damage to the endothelium and nearby tissue. Protective mechanisms exist; however, these systems are not sufficient in diseases with increased extravascular hemolysis e.g. hemolytic anemia. α1-microglobulin (A1M) is a ubiquitous reductase and radical- and heme-binding protein with antioxidation properties. Althou

Efficient and nontoxic biomolecule delivery to primary human hematopoietic stem cells using nanostraws

Introduction of exogenous genetic material into primary stem cells is essential for studying biological function and for clinical applications. Traditional delivery methods for nucleic acids, such as electroporation, have advanced the field, but have negative effects on stem cell function and viability. We introduce nanostraw-assisted transfection as an alternative method for RNA delivery to human

Acoustophoresis enables the label-free separation of functionally different subsets of cultured bone marrow stromal cells

Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell-based therapies. MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound-based separation technology, could be used for the label-free enrichment of functionally different MSC populations. Acoustophoresis uses an ultrasonic standing wave in a microchannel which d